Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14
Bacteria with the ability to tolerate, remove, and/or degrade several xenobiotics simultaneously are urgently needed for remediation of polluted sites. A previously isolated bacterium with sodium dodecyl sulfate- (SDS-) degrading capacity was found to be able to reduce molybdenum to the nontoxic mol...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28091/ http://psasir.upm.edu.my/id/eprint/28091/1/28091.pdf |
| _version_ | 1848846020295786496 |
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| author | Halmi, Mohd Izuan Effendi Saad, Wan Zuhainis Yusof, Mohd Termizi Shaharuddin, Noor Azmi Wasoh @ Mohamad Isa, Helmi Abd. Shukor, Mohd. Yunus Syed, Mohd Arif Ahmad, Siti Aqlima |
| author_facet | Halmi, Mohd Izuan Effendi Saad, Wan Zuhainis Yusof, Mohd Termizi Shaharuddin, Noor Azmi Wasoh @ Mohamad Isa, Helmi Abd. Shukor, Mohd. Yunus Syed, Mohd Arif Ahmad, Siti Aqlima |
| author_sort | Halmi, Mohd Izuan Effendi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacteria with the ability to tolerate, remove, and/or degrade several xenobiotics simultaneously are urgently needed for remediation of polluted sites. A previously isolated bacterium with sodium dodecyl sulfate- (SDS-) degrading capacity was found to be able to reduce molybdenum to the nontoxic molybdenum blue. The optimal pH, carbon source, molybdate concentration, and temperature supporting molybdate reduction were pH 7.0, glucose at 1.5% (w/v), between 25 and 30 mM, and 25°C, respectively. The optimum phosphate concentration for molybdate reduction was 5 mM. The Mo-blue produced exhibits an absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. None of the respiratory inhibitors tested showed any inhibition to the molybdenum-reducing activity suggesting that the electron transport system of this bacterium is not the site of molybdenum reduction. Chromium, cadmium, silver, copper, mercury, and lead caused approximately 77, 65, 77, 89, 80, and 80% inhibition of the molybdenum-reducing activity, respectively. Ferrous and stannous ions markedly increased the activity of molybdenum-reducing activity in this bacterium. The maximum tolerable concentration of SDS as a cocontaminant was 3 g/L. The characteristics of this bacterium make it a suitable candidate for molybdenum bioremediation of sites cocontaminated with detergent pollutant. |
| first_indexed | 2025-11-15T08:56:04Z |
| format | Article |
| id | upm-28091 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:56:04Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280912016-06-20T06:12:37Z http://psasir.upm.edu.my/id/eprint/28091/ Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 Halmi, Mohd Izuan Effendi Saad, Wan Zuhainis Yusof, Mohd Termizi Shaharuddin, Noor Azmi Wasoh @ Mohamad Isa, Helmi Abd. Shukor, Mohd. Yunus Syed, Mohd Arif Ahmad, Siti Aqlima Bacteria with the ability to tolerate, remove, and/or degrade several xenobiotics simultaneously are urgently needed for remediation of polluted sites. A previously isolated bacterium with sodium dodecyl sulfate- (SDS-) degrading capacity was found to be able to reduce molybdenum to the nontoxic molybdenum blue. The optimal pH, carbon source, molybdate concentration, and temperature supporting molybdate reduction were pH 7.0, glucose at 1.5% (w/v), between 25 and 30 mM, and 25°C, respectively. The optimum phosphate concentration for molybdate reduction was 5 mM. The Mo-blue produced exhibits an absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. None of the respiratory inhibitors tested showed any inhibition to the molybdenum-reducing activity suggesting that the electron transport system of this bacterium is not the site of molybdenum reduction. Chromium, cadmium, silver, copper, mercury, and lead caused approximately 77, 65, 77, 89, 80, and 80% inhibition of the molybdenum-reducing activity, respectively. Ferrous and stannous ions markedly increased the activity of molybdenum-reducing activity in this bacterium. The maximum tolerable concentration of SDS as a cocontaminant was 3 g/L. The characteristics of this bacterium make it a suitable candidate for molybdenum bioremediation of sites cocontaminated with detergent pollutant. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28091/1/28091.pdf Halmi, Mohd Izuan Effendi and Saad, Wan Zuhainis and Yusof, Mohd Termizi and Shaharuddin, Noor Azmi and Wasoh @ Mohamad Isa, Helmi and Abd. Shukor, Mohd. Yunus and Syed, Mohd Arif and Ahmad, Siti Aqlima (2013) Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14. BioMed Research International, 2013. art. no. 384541. pp. 1-8. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/384541/abs/ 10.1155/2013/384541 |
| spellingShingle | Halmi, Mohd Izuan Effendi Saad, Wan Zuhainis Yusof, Mohd Termizi Shaharuddin, Noor Azmi Wasoh @ Mohamad Isa, Helmi Abd. Shukor, Mohd. Yunus Syed, Mohd Arif Ahmad, Siti Aqlima Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title | Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title_full | Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title_fullStr | Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title_full_unstemmed | Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title_short | Hexavalent molybdenum reduction to Mo-Blue by a sodium-dodecyl-sulfate-degrading Klebsiella oxytoca strain DRY14 |
| title_sort | hexavalent molybdenum reduction to mo-blue by a sodium-dodecyl-sulfate-degrading klebsiella oxytoca strain dry14 |
| url | http://psasir.upm.edu.my/id/eprint/28091/ http://psasir.upm.edu.my/id/eprint/28091/ http://psasir.upm.edu.my/id/eprint/28091/ http://psasir.upm.edu.my/id/eprint/28091/1/28091.pdf |