Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum

Coconut oil is a rich source of beneficial medium chain fatty acids (MCFAs) particularly lauric acid. In this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (DIMOSFER) method. A coconut-based and coconut-oil-added solid-state cultiv...

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Main Authors: Khoramnia, Anahita, Ebrahimpour, Afshin, Ghanbari, Raheleh, Ajdari, Zahra, Lai, Oi Ming
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28082/
http://psasir.upm.edu.my/id/eprint/28082/1/954542.pdf
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author Khoramnia, Anahita
Ebrahimpour, Afshin
Ghanbari, Raheleh
Ajdari, Zahra
Lai, Oi Ming
author_facet Khoramnia, Anahita
Ebrahimpour, Afshin
Ghanbari, Raheleh
Ajdari, Zahra
Lai, Oi Ming
author_sort Khoramnia, Anahita
building UPM Institutional Repository
collection Online Access
description Coconut oil is a rich source of beneficial medium chain fatty acids (MCFAs) particularly lauric acid. In this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (DIMOSFER) method. A coconut-based and coconut-oil-added solid-state cultivation using a Malaysian lipolytic Geotrichum candidum was used to convert the coconut oil into MCFAs-rich oil. Chemical characteristics of the modified coconut oils (MCOs) considering total medium chain glyceride esters were compared to those of the normal coconut oil using ELSD-RP-HPLC. Optimum amount of coconut oil hydrolysis was achieved at 29% moisture content and 10.14% oil content after 9 days of incubation, where the quantitative amounts of the modified coconut oil and MCFA were 0.330?mL/g of solid media (76.5% bioconversion) and 0.175?mL/g of solid media (53% of the MCO), respectively. MCOs demonstrated improved antibacterial activity mostly due to the presence of free lauric acid. The highest MCFAs-rich coconut oil revealed as much as 90% and 80% antibacterial activities against Staphylococcus aureus and Escherichia coli, respectively. The results of the study showed that DIMOSFER by a local lipolytic G. candidum can be used to produce MCFAs as natural, effective, and safe antimicrobial agent. The produced MCOs and MCFAs could be further applied in food and pharmaceutical industries.
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spelling upm-280822016-04-22T02:40:55Z http://psasir.upm.edu.my/id/eprint/28082/ Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum Khoramnia, Anahita Ebrahimpour, Afshin Ghanbari, Raheleh Ajdari, Zahra Lai, Oi Ming Coconut oil is a rich source of beneficial medium chain fatty acids (MCFAs) particularly lauric acid. In this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (DIMOSFER) method. A coconut-based and coconut-oil-added solid-state cultivation using a Malaysian lipolytic Geotrichum candidum was used to convert the coconut oil into MCFAs-rich oil. Chemical characteristics of the modified coconut oils (MCOs) considering total medium chain glyceride esters were compared to those of the normal coconut oil using ELSD-RP-HPLC. Optimum amount of coconut oil hydrolysis was achieved at 29% moisture content and 10.14% oil content after 9 days of incubation, where the quantitative amounts of the modified coconut oil and MCFA were 0.330?mL/g of solid media (76.5% bioconversion) and 0.175?mL/g of solid media (53% of the MCO), respectively. MCOs demonstrated improved antibacterial activity mostly due to the presence of free lauric acid. The highest MCFAs-rich coconut oil revealed as much as 90% and 80% antibacterial activities against Staphylococcus aureus and Escherichia coli, respectively. The results of the study showed that DIMOSFER by a local lipolytic G. candidum can be used to produce MCFAs as natural, effective, and safe antimicrobial agent. The produced MCOs and MCFAs could be further applied in food and pharmaceutical industries. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28082/1/954542.pdf Khoramnia, Anahita and Ebrahimpour, Afshin and Ghanbari, Raheleh and Ajdari, Zahra and Lai, Oi Ming (2013) Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum. BioMed Research International, 2013. art. no. 954542. pp. 1-9. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/954542/abs/ 10.1155/2013/954542
spellingShingle Khoramnia, Anahita
Ebrahimpour, Afshin
Ghanbari, Raheleh
Ajdari, Zahra
Lai, Oi Ming
Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title_full Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title_fullStr Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title_full_unstemmed Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title_short Improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a Malaysian Geotrichum candidum
title_sort improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a malaysian geotrichum candidum
url http://psasir.upm.edu.my/id/eprint/28082/
http://psasir.upm.edu.my/id/eprint/28082/
http://psasir.upm.edu.my/id/eprint/28082/
http://psasir.upm.edu.my/id/eprint/28082/1/954542.pdf