Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli
Objective: Lipases are industrially important enzymes especially the thermostable and organic solvent tolerant lipases. This study was planned to enhance the production using fed batch fermentation technique. Materials and Methods: The possibility of using fed-batch fermentation for improvement of o...
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| Format: | Article |
| Language: | English |
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Turkish Biochemical Society
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28080/ http://psasir.upm.edu.my/id/eprint/28080/1/Optimization%20of%20fed-batch%20fermentation%20for%20organic%20solvent%20tolerant%20and%20thermostable%20lipase%20production%20from%20recombinant%20E.%20coli.pdf |
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| author | Nelofer, Rubina Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya |
| author_facet | Nelofer, Rubina Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya |
| author_sort | Nelofer, Rubina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: Lipases are industrially important enzymes especially the thermostable and organic solvent tolerant lipases. This study was planned to enhance the production using fed batch fermentation technique. Materials and Methods: The possibility of using fed-batch fermentation for improvement of organic solvent tolerant and thermostable lipase production by recombinant E.coli BL21 was studied in 2 L stirred tank bioreactor. Exponential fed-batch fermentations were operated at different specific growth rates (μ). The effect of antibiotics addition in feed medium, on the number of plasmid bearing cells was also investigated. Kinetics of the fed-batch fermentation was evaluated for the generation of kinetic parameter values. Effect of yeast extract in feed medium was also investigated. Results: The number of plasmid bearing cells was increased from 13 % to 91 % with the addition of antibiotics (2 g/L ampicillin and 1.4 g/L chloramphenicol) in the feed medium. Among the different μ investigated (0.05, 0.10 and 0.15 h-1), the highest cell concentration (30.32 g/L) and lipase production (130.5 IU/mL) were obtained in exponential fed-batch fermentation, where μ was controlled at 0.10 h-1 with 800 g/L glucose in the feed medium. No significant improvement was achieved on growth and lipase production in fed-batch fermentation with addition of yeast extract in feed medium, though μ was controlled at 0.10 h-1. Conclusion: Fed-batch was found a good technique for enhancement of lipase production from recombinant E.coli BL21 on the cost of increasing time of fermentation (fermentation time for batch fermentation was 16 h and for fed-batch was 26 h) and quantity of some medium ingredients as compared to the batch mode. |
| first_indexed | 2025-11-15T08:56:01Z |
| format | Article |
| id | upm-28080 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:56:01Z |
| publishDate | 2013 |
| publisher | Turkish Biochemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280802016-06-20T03:06:48Z http://psasir.upm.edu.my/id/eprint/28080/ Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli Nelofer, Rubina Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya Objective: Lipases are industrially important enzymes especially the thermostable and organic solvent tolerant lipases. This study was planned to enhance the production using fed batch fermentation technique. Materials and Methods: The possibility of using fed-batch fermentation for improvement of organic solvent tolerant and thermostable lipase production by recombinant E.coli BL21 was studied in 2 L stirred tank bioreactor. Exponential fed-batch fermentations were operated at different specific growth rates (μ). The effect of antibiotics addition in feed medium, on the number of plasmid bearing cells was also investigated. Kinetics of the fed-batch fermentation was evaluated for the generation of kinetic parameter values. Effect of yeast extract in feed medium was also investigated. Results: The number of plasmid bearing cells was increased from 13 % to 91 % with the addition of antibiotics (2 g/L ampicillin and 1.4 g/L chloramphenicol) in the feed medium. Among the different μ investigated (0.05, 0.10 and 0.15 h-1), the highest cell concentration (30.32 g/L) and lipase production (130.5 IU/mL) were obtained in exponential fed-batch fermentation, where μ was controlled at 0.10 h-1 with 800 g/L glucose in the feed medium. No significant improvement was achieved on growth and lipase production in fed-batch fermentation with addition of yeast extract in feed medium, though μ was controlled at 0.10 h-1. Conclusion: Fed-batch was found a good technique for enhancement of lipase production from recombinant E.coli BL21 on the cost of increasing time of fermentation (fermentation time for batch fermentation was 16 h and for fed-batch was 26 h) and quantity of some medium ingredients as compared to the batch mode. Turkish Biochemical Society 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28080/1/Optimization%20of%20fed-batch%20fermentation%20for%20organic%20solvent%20tolerant%20and%20thermostable%20lipase%20production%20from%20recombinant%20E.%20coli.pdf Nelofer, Rubina and Raja Abdul Rahman, Raja Noor Zaliha and Basri, Mahiran and Ariff, Arbakariya (2013) Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli. Turkish Journal of Biochemistry, 38 (3). pp. 299-307. ISSN 0250-4685; ESSN: 1303-829X http://www.turkjbiochem.com/2013/toc.html 10.5505/tjb.2013.81994 |
| spellingShingle | Nelofer, Rubina Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title | Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title_full | Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title_fullStr | Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title_full_unstemmed | Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title_short | Optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant E. coli |
| title_sort | optimization of fed-batch fermentation for organic solvent tolerant and thermostable lipase production from recombinant e. coli |
| url | http://psasir.upm.edu.my/id/eprint/28080/ http://psasir.upm.edu.my/id/eprint/28080/ http://psasir.upm.edu.my/id/eprint/28080/ http://psasir.upm.edu.my/id/eprint/28080/1/Optimization%20of%20fed-batch%20fermentation%20for%20organic%20solvent%20tolerant%20and%20thermostable%20lipase%20production%20from%20recombinant%20E.%20coli.pdf |