Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil
A bacterium capable of biodegrading surfactant sodium dodecyl sulphate (SDS) was isolated from Antarctic soil. The isolate was tentatively identified as Pseudomonas sp. strain DRY15 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Growth character...
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Triveni Enterprises
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28075/ http://psasir.upm.edu.my/id/eprint/28075/1/28075.pdf |
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| author | Halmi, Mohd Izuan Effendi W. Hussin, W. S. Ahmad, Siti Aqlima Syed, Mohd Arif Ruberto, Lucas MacCormack, Walter P. Abd. Shukor, Mohd. Yunus |
| author_facet | Halmi, Mohd Izuan Effendi W. Hussin, W. S. Ahmad, Siti Aqlima Syed, Mohd Arif Ruberto, Lucas MacCormack, Walter P. Abd. Shukor, Mohd. Yunus |
| author_sort | Halmi, Mohd Izuan Effendi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A bacterium capable of biodegrading surfactant sodium dodecyl sulphate (SDS) was isolated from Antarctic soil. The isolate was tentatively identified as Pseudomonas sp. strain DRY15 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Growth characteristic studies showed that the bacterium grew optimally at 10 degrees C, 7.25 pH, 1 g l(-1) SDS as a sole carbon source and 2 g l(-1) ammonium sulphate as nitrogen source. Growth was completely inhibited at 5 g l(-1) SDS. At a tolerable initial concentration of 2 g l(-1), approximately 90% of SDS was degraded after an incubation period of eight days. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibition with a correlation coefficient value of 0.97. The maximum growth rate was 0.372 hr(-1) while the saturation constant or half velocity constant (Ks) and inhibition constant (Ki), were 0.094% and 11.212 % SDS, respectively. Other detergent tested as carbon sources at 1 g l(-1) was Tergitol NP9, Tergitol 15S9, Witconol 2301 (methyl oleate), sodium dodecylbenzene sulfonate (SDBS), benzethonium chloride, and benzalkonium chloride showed Tergitol NP9, Tergitol 15S9, Witconol 2301 and the anionic SDBS supported growth with the highest growth exhibited by SDBS. |
| first_indexed | 2025-11-15T08:55:59Z |
| format | Article |
| id | upm-28075 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:59Z |
| publishDate | 2013 |
| publisher | Triveni Enterprises |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280752016-04-22T07:48:25Z http://psasir.upm.edu.my/id/eprint/28075/ Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil Halmi, Mohd Izuan Effendi W. Hussin, W. S. Ahmad, Siti Aqlima Syed, Mohd Arif Ruberto, Lucas MacCormack, Walter P. Abd. Shukor, Mohd. Yunus A bacterium capable of biodegrading surfactant sodium dodecyl sulphate (SDS) was isolated from Antarctic soil. The isolate was tentatively identified as Pseudomonas sp. strain DRY15 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Growth characteristic studies showed that the bacterium grew optimally at 10 degrees C, 7.25 pH, 1 g l(-1) SDS as a sole carbon source and 2 g l(-1) ammonium sulphate as nitrogen source. Growth was completely inhibited at 5 g l(-1) SDS. At a tolerable initial concentration of 2 g l(-1), approximately 90% of SDS was degraded after an incubation period of eight days. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibition with a correlation coefficient value of 0.97. The maximum growth rate was 0.372 hr(-1) while the saturation constant or half velocity constant (Ks) and inhibition constant (Ki), were 0.094% and 11.212 % SDS, respectively. Other detergent tested as carbon sources at 1 g l(-1) was Tergitol NP9, Tergitol 15S9, Witconol 2301 (methyl oleate), sodium dodecylbenzene sulfonate (SDBS), benzethonium chloride, and benzalkonium chloride showed Tergitol NP9, Tergitol 15S9, Witconol 2301 and the anionic SDBS supported growth with the highest growth exhibited by SDBS. Triveni Enterprises 2013-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28075/1/28075.pdf Halmi, Mohd Izuan Effendi and W. Hussin, W. S. and Ahmad, Siti Aqlima and Syed, Mohd Arif and Ruberto, Lucas and MacCormack, Walter P. and Abd. Shukor, Mohd. Yunus (2013) Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil. Journal of Environmental Biology, 34 (6). pp. 1077-1082. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=201311_nov13&number=18 |
| spellingShingle | Halmi, Mohd Izuan Effendi W. Hussin, W. S. Ahmad, Siti Aqlima Syed, Mohd Arif Ruberto, Lucas MacCormack, Walter P. Abd. Shukor, Mohd. Yunus Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title | Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title_full | Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title_fullStr | Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title_full_unstemmed | Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title_short | Characterization of a sodium dodecyl sulphate-degrading Pseudomonas sp. strain DRY15 from Antarctic soil |
| title_sort | characterization of a sodium dodecyl sulphate-degrading pseudomonas sp. strain dry15 from antarctic soil |
| url | http://psasir.upm.edu.my/id/eprint/28075/ http://psasir.upm.edu.my/id/eprint/28075/ http://psasir.upm.edu.my/id/eprint/28075/1/28075.pdf |