Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis
Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominan...
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28071/ http://psasir.upm.edu.my/id/eprint/28071/1/Display%20of%20the%20VP1%20epitope%20of%20foot-and-mouth%20disease%20virus%20on%20bacteriophage%20T7%20and%20its%20application%20in%20diagnosis.pdf |
| _version_ | 1848846014147985408 |
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| author | Wong, Chuan Loo Sieo, Chin Chin Tan, Wen Siang |
| author_facet | Wong, Chuan Loo Sieo, Chin Chin Tan, Wen Siang |
| author_sort | Wong, Chuan Loo |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominant region of the capsid protein VP1 of the foot-and-mouth disease virus (FMDV) was fused to the T7 bacteriophage and expressed on the surface of the bacteriophage capsid protein. The recombinant protein of about 42 kDa was detected by the anti-T7 tag monoclonal antibody in Western blot analysis. Phage ELISA showed that both the vaccinated and positive infected bovine sera reacted significantly with the recombinant T7 particle. This study demonstrated the potential of the T7 phage displaying the VP1 epitope as a diagnostic reagent. |
| first_indexed | 2025-11-15T08:55:58Z |
| format | Article |
| id | upm-28071 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:58Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280712016-04-21T05:59:03Z http://psasir.upm.edu.my/id/eprint/28071/ Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis Wong, Chuan Loo Sieo, Chin Chin Tan, Wen Siang Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominant region of the capsid protein VP1 of the foot-and-mouth disease virus (FMDV) was fused to the T7 bacteriophage and expressed on the surface of the bacteriophage capsid protein. The recombinant protein of about 42 kDa was detected by the anti-T7 tag monoclonal antibody in Western blot analysis. Phage ELISA showed that both the vaccinated and positive infected bovine sera reacted significantly with the recombinant T7 particle. This study demonstrated the potential of the T7 phage displaying the VP1 epitope as a diagnostic reagent. Elsevier 2013-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28071/1/Display%20of%20the%20VP1%20epitope%20of%20foot-and-mouth%20disease%20virus%20on%20bacteriophage%20T7%20and%20its%20application%20in%20diagnosis.pdf Wong, Chuan Loo and Sieo, Chin Chin and Tan, Wen Siang (2013) Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis. Journal of Virological Methods, 193 (2). pp. 611-619. ISSN 0166-0934; ESSN: 1879-0984 http://www.elsevier.com/locate/jviromet 10.1016/j.jviromet.2013.07.053 |
| spellingShingle | Wong, Chuan Loo Sieo, Chin Chin Tan, Wen Siang Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title | Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title_full | Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title_fullStr | Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title_full_unstemmed | Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title_short | Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis |
| title_sort | display of the vp1 epitope of foot-and-mouth disease virus on bacteriophage t7 and its application in diagnosis |
| url | http://psasir.upm.edu.my/id/eprint/28071/ http://psasir.upm.edu.my/id/eprint/28071/ http://psasir.upm.edu.my/id/eprint/28071/ http://psasir.upm.edu.my/id/eprint/28071/1/Display%20of%20the%20VP1%20epitope%20of%20foot-and-mouth%20disease%20virus%20on%20bacteriophage%20T7%20and%20its%20application%20in%20diagnosis.pdf |