Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography
NPΔc375 is a truncated version of the nucleocapsid protein of Newcastle disease virus (NDV) which self-assembles into a long helical structure. A packed bed anion exchange chromatography (PB-AEC), SepFastTM Supor Q pre-packed column, was used to purify NPΔc375 from clarified feedstock. This PB-AEC c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Institute of Chemical Engineers
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28069/ http://psasir.upm.edu.my/id/eprint/28069/1/28069.pdf |
| _version_ | 1848846013658300416 |
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| author | Yap, Chee Fai Tan, Wen Siang Sieo, Chin Chin Tey, Beng Ti |
| author_facet | Yap, Chee Fai Tan, Wen Siang Sieo, Chin Chin Tey, Beng Ti |
| author_sort | Yap, Chee Fai |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | NPΔc375 is a truncated version of the nucleocapsid protein of Newcastle disease virus (NDV) which self-assembles into a long helical structure. A packed bed anion exchange chromatography (PB-AEC), SepFastTM Supor Q pre-packed column, was used to purify NPΔc375 from clarified feedstock. This PB-AEC column adsorbed 76.2% of NPΔc375 from the clarified feedstock. About 67.5% of the adsorbed NPΔc375 was successfully eluted from the column by applying 50 mM Tris-HCl elution buffer supplemented with 0.5 M NaCl at pH 7. Thus, a recovery yield of 51.4% with a purity of 76.7% which corresponds to a purification factor of 6.5 was achieved in this PB-AEC operation. Electron microscopic analysis revealed that the helical structure of the NPΔc375 purified by SepFastTM Supor Q pre-packed column was as long as 490 nm and 22–24 nm in diameter. The antigenicity of the purified NPΔc375 was confirmed by enzyme-linked immunosorbent assay. |
| first_indexed | 2025-11-15T08:55:58Z |
| format | Article |
| id | upm-28069 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:58Z |
| publishDate | 2013 |
| publisher | American Institute of Chemical Engineers |
| recordtype | eprints |
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| spelling | upm-280692016-04-21T06:06:27Z http://psasir.upm.edu.my/id/eprint/28069/ Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography Yap, Chee Fai Tan, Wen Siang Sieo, Chin Chin Tey, Beng Ti NPΔc375 is a truncated version of the nucleocapsid protein of Newcastle disease virus (NDV) which self-assembles into a long helical structure. A packed bed anion exchange chromatography (PB-AEC), SepFastTM Supor Q pre-packed column, was used to purify NPΔc375 from clarified feedstock. This PB-AEC column adsorbed 76.2% of NPΔc375 from the clarified feedstock. About 67.5% of the adsorbed NPΔc375 was successfully eluted from the column by applying 50 mM Tris-HCl elution buffer supplemented with 0.5 M NaCl at pH 7. Thus, a recovery yield of 51.4% with a purity of 76.7% which corresponds to a purification factor of 6.5 was achieved in this PB-AEC operation. Electron microscopic analysis revealed that the helical structure of the NPΔc375 purified by SepFastTM Supor Q pre-packed column was as long as 490 nm and 22–24 nm in diameter. The antigenicity of the purified NPΔc375 was confirmed by enzyme-linked immunosorbent assay. American Institute of Chemical Engineers 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28069/1/28069.pdf Yap, Chee Fai and Tan, Wen Siang and Sieo, Chin Chin and Tey, Beng Ti (2013) Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography. Biotechnology Progress, 29 (2). pp. 564-567. ISSN 8756-7938; ESSN: 1520-6033 http://onlinelibrary.wiley.com/wol1/doi/10.1002/btpr.1697/abstract 10.1002/btpr.1697 |
| spellingShingle | Yap, Chee Fai Tan, Wen Siang Sieo, Chin Chin Tey, Beng Ti Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title | Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title_full | Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title_fullStr | Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title_full_unstemmed | Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title_short | Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography |
| title_sort | purification of long helical capsid of newcastle disease virus from escherichia coli using anion exchange chromatography |
| url | http://psasir.upm.edu.my/id/eprint/28069/ http://psasir.upm.edu.my/id/eprint/28069/ http://psasir.upm.edu.my/id/eprint/28069/ http://psasir.upm.edu.my/id/eprint/28069/1/28069.pdf |