Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions
The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28065/ http://psasir.upm.edu.my/id/eprint/28065/1/Cell%20viability%20of%20microencapsulated%20Bifidobacterium%20animalis%20subsp.pdf |
| _version_ | 1848846012547858432 |
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| author | Shamekhi, Fatemeh Shuhaimi, Mustafa Ariff, Arbakariya Abdul Manap, Yazid |
| author_facet | Shamekhi, Fatemeh Shuhaimi, Mustafa Ariff, Arbakariya Abdul Manap, Yazid |
| author_sort | Shamekhi, Fatemeh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics–calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical. |
| first_indexed | 2025-11-15T08:55:57Z |
| format | Article |
| id | upm-28065 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:57Z |
| publishDate | 2013 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280652017-12-08T04:34:50Z http://psasir.upm.edu.my/id/eprint/28065/ Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions Shamekhi, Fatemeh Shuhaimi, Mustafa Ariff, Arbakariya Abdul Manap, Yazid The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics–calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical. Springer Netherlands 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28065/1/Cell%20viability%20of%20microencapsulated%20Bifidobacterium%20animalis%20subsp.pdf Shamekhi, Fatemeh and Shuhaimi, Mustafa and Ariff, Arbakariya and Abdul Manap, Yazid (2013) Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions. Folia Microbiologica, 58 (2013). pp. 91-101. ISSN 0015-5632; ESSN: 1874-9356 http://link.springer.com/article/10.1007%2Fs12223-012-0183-9 10.1007/s12223-012-0183-9 |
| spellingShingle | Shamekhi, Fatemeh Shuhaimi, Mustafa Ariff, Arbakariya Abdul Manap, Yazid Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title | Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title_full | Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title_fullStr | Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title_full_unstemmed | Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title_short | Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| title_sort | cell viability of microencapsulated bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions |
| url | http://psasir.upm.edu.my/id/eprint/28065/ http://psasir.upm.edu.my/id/eprint/28065/ http://psasir.upm.edu.my/id/eprint/28065/ http://psasir.upm.edu.my/id/eprint/28065/1/Cell%20viability%20of%20microencapsulated%20Bifidobacterium%20animalis%20subsp.pdf |