Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review
Pork adulteration has been a major concern nowadays for Halal verification. Unintentional pork inclusion by contamination in highly processed food materials involves a minute amount of porcine DNA to be detected, emphasizing the need of specific and sensitive method for porcine detection. Real-time...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28064/ http://psasir.upm.edu.my/id/eprint/28064/1/Comparison%20of%20gene%20nature%20used%20in%20real-time%20PCR%20for%20porcine%20identification%20and%20quantification%20a%20review.pdf |
| _version_ | 1848846012288860160 |
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| author | Mohamad, Nurhidayatul Asma El Sheikha, Aly Farag Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah |
| author_facet | Mohamad, Nurhidayatul Asma El Sheikha, Aly Farag Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah |
| author_sort | Mohamad, Nurhidayatul Asma |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pork adulteration has been a major concern nowadays for Halal verification. Unintentional pork inclusion by contamination in highly processed food materials involves a minute amount of porcine DNA to be detected, emphasizing the need of specific and sensitive method for porcine detection. Real-time PCR is a widely used technique for species identification that can serve this purpose besides providing a powerful quantification method. Incorporation of a highly sensitive and specific probe can greatly improve the specificity and sensitivity of the assay. However, derivation of PCR primers, either from nuclear DNA (nDNA) or mitochondrial DNA (mtDNA) can relatively affect the sensitivity and specificity of the reaction as well as the quantitative measurement. In this review, both types of DNA are compared in terms of their characteristics and their influence on species identification and quantification using real-time PCR. |
| first_indexed | 2025-11-15T08:55:56Z |
| format | Article |
| id | upm-28064 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:56Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280642016-04-21T04:28:27Z http://psasir.upm.edu.my/id/eprint/28064/ Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review Mohamad, Nurhidayatul Asma El Sheikha, Aly Farag Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Pork adulteration has been a major concern nowadays for Halal verification. Unintentional pork inclusion by contamination in highly processed food materials involves a minute amount of porcine DNA to be detected, emphasizing the need of specific and sensitive method for porcine detection. Real-time PCR is a widely used technique for species identification that can serve this purpose besides providing a powerful quantification method. Incorporation of a highly sensitive and specific probe can greatly improve the specificity and sensitivity of the assay. However, derivation of PCR primers, either from nuclear DNA (nDNA) or mitochondrial DNA (mtDNA) can relatively affect the sensitivity and specificity of the reaction as well as the quantitative measurement. In this review, both types of DNA are compared in terms of their characteristics and their influence on species identification and quantification using real-time PCR. Elsevier 2013-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28064/1/Comparison%20of%20gene%20nature%20used%20in%20real-time%20PCR%20for%20porcine%20identification%20and%20quantification%20a%20review.pdf Mohamad, Nurhidayatul Asma and El Sheikha, Aly Farag and Mustafa, Shuhaimi and Khairil Mokhtar, Nur Fadhilah (2013) Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review. Food Research International, 50 (1). pp. 330-338. ISSN 0963-9969; ESSN: 1873-7145 10.1016/j.foodres.2012.10.047 |
| spellingShingle | Mohamad, Nurhidayatul Asma El Sheikha, Aly Farag Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title | Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title_full | Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title_fullStr | Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title_full_unstemmed | Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title_short | Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review |
| title_sort | comparison of gene nature used in real-time pcr for porcine identification and quantification: a review |
| url | http://psasir.upm.edu.my/id/eprint/28064/ http://psasir.upm.edu.my/id/eprint/28064/ http://psasir.upm.edu.my/id/eprint/28064/1/Comparison%20of%20gene%20nature%20used%20in%20real-time%20PCR%20for%20porcine%20identification%20and%20quantification%20a%20review.pdf |