Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain
Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm fr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28063/ http://psasir.upm.edu.my/id/eprint/28063/1/Biohydrogen%20production%20from%20oil%20palm%20frond%20juice%20and%20sewage%20sludge%20by%20a%20metabolically%20engineered%20Escherichia%20coli%20strain.pdf |
| _version_ | 1848846012056076288 |
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| author | Mohd Yasin, Nazlina Haiza Fukuzaki, Masaharu Maeda, Toshinari Miyazaki, Toshiki Che Maail, Che Mohd Hakiman Ariffin, Hidayah Wood, Thomas K. |
| author_facet | Mohd Yasin, Nazlina Haiza Fukuzaki, Masaharu Maeda, Toshinari Miyazaki, Toshiki Che Maail, Che Mohd Hakiman Ariffin, Hidayah Wood, Thomas K. |
| author_sort | Mohd Yasin, Nazlina Haiza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm frond (OPF) juice and sewage sludge as substrates. Substrate improvement was accomplished when hydrogen productivity increased 8-fold after enzymatic treatment of the sludge with a mixture of amylase and cellulase. The OPF juice with sewage sludge provided an optimum carbon/nitrogen ratio since the yield of biohydrogen increased to 1.5 from 1.3 mol H2/mol glucose compared to our previous study. In this study, we also reveal that our engineered strain improved 200-fold biohydrogen productivity from biomass sources compared to the unmodified host. In conclusion, we determined that our engineered strain can use biomass as an alternative substrate for enhanced biohydrogen production. |
| first_indexed | 2025-11-15T08:55:56Z |
| format | Article |
| id | upm-28063 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:56Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280632016-04-21T05:39:53Z http://psasir.upm.edu.my/id/eprint/28063/ Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain Mohd Yasin, Nazlina Haiza Fukuzaki, Masaharu Maeda, Toshinari Miyazaki, Toshiki Che Maail, Che Mohd Hakiman Ariffin, Hidayah Wood, Thomas K. Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm frond (OPF) juice and sewage sludge as substrates. Substrate improvement was accomplished when hydrogen productivity increased 8-fold after enzymatic treatment of the sludge with a mixture of amylase and cellulase. The OPF juice with sewage sludge provided an optimum carbon/nitrogen ratio since the yield of biohydrogen increased to 1.5 from 1.3 mol H2/mol glucose compared to our previous study. In this study, we also reveal that our engineered strain improved 200-fold biohydrogen productivity from biomass sources compared to the unmodified host. In conclusion, we determined that our engineered strain can use biomass as an alternative substrate for enhanced biohydrogen production. Elsevier 2013-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28063/1/Biohydrogen%20production%20from%20oil%20palm%20frond%20juice%20and%20sewage%20sludge%20by%20a%20metabolically%20engineered%20Escherichia%20coli%20strain.pdf Mohd Yasin, Nazlina Haiza and Fukuzaki, Masaharu and Maeda, Toshinari and Miyazaki, Toshiki and Che Maail, Che Mohd Hakiman and Ariffin, Hidayah and Wood, Thomas K. (2013) Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain. International Journal of Hydrogen Energy, 38 (25). pp. 10277-10283. ISSN 0360-3199; ESSN: 1879-3487 10.1016/j.ijhydene.2013.06.065 |
| spellingShingle | Mohd Yasin, Nazlina Haiza Fukuzaki, Masaharu Maeda, Toshinari Miyazaki, Toshiki Che Maail, Che Mohd Hakiman Ariffin, Hidayah Wood, Thomas K. Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title | Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title_full | Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title_fullStr | Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title_full_unstemmed | Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title_short | Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain |
| title_sort | biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered escherichia coli strain |
| url | http://psasir.upm.edu.my/id/eprint/28063/ http://psasir.upm.edu.my/id/eprint/28063/ http://psasir.upm.edu.my/id/eprint/28063/1/Biohydrogen%20production%20from%20oil%20palm%20frond%20juice%20and%20sewage%20sludge%20by%20a%20metabolically%20engineered%20Escherichia%20coli%20strain.pdf |