Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy me...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Parlar Scientific Publications
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28046/ http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf |
| _version_ | 1848846006849896448 |
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| author | Aidil, Mohd S. Sabullah, Mohd Khalidan Halmi, Mohd Izuan Effendi Sulaiman, Rosni Shukor, Mohd Shukri Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Syed, Mohd Arif Amir Hamzah, Amir Syahir |
| author_facet | Aidil, Mohd S. Sabullah, Mohd Khalidan Halmi, Mohd Izuan Effendi Sulaiman, Rosni Shukor, Mohd Shukri Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Syed, Mohd Arif Amir Hamzah, Amir Syahir |
| author_sort | Aidil, Mohd S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy metals. We discovered that the AChE was completely inhibited by Cr6+, Ag 2+, Cd2+, Hg2+, Cu2+, Zn 2+ and Pb2+ during an initial screening. When testing at various concentrations, the above heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for Hg2+, Cu 2+, Ag , Cr6+, Cd2+, Pb2+, and Zn2+were 0.071, 0.088, 0.088, 0.87, 0.913, 1.1, and 1.572 mg/L, respectively. The Limit of Detection LOD and Limit of Quantification LOQ values of the heavy metals are within the range of the Maximum Permissibility Limit MPL allowed by the Malaysian Department of Environment DOE for Class-Ill rivers. The IC50 values for these heavy metals are comparable, and some are lower than those of the currently known assays, such as immobilised urease, Daphnia magna, Microtox™, rainbow trout, papain and bromelain assays. Field trial works on an industrial drainage site had shown that the developed assay is applicable in detecting heavy metals in the water. The assay can be carried out in less than 30 min under ambient temperature. |
| first_indexed | 2025-11-15T08:55:51Z |
| format | Article |
| id | upm-28046 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:51Z |
| publishDate | 2013 |
| publisher | Parlar Scientific Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280462016-04-25T07:11:30Z http://psasir.upm.edu.my/id/eprint/28046/ Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) Aidil, Mohd S. Sabullah, Mohd Khalidan Halmi, Mohd Izuan Effendi Sulaiman, Rosni Shukor, Mohd Shukri Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Syed, Mohd Arif Amir Hamzah, Amir Syahir Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy metals. We discovered that the AChE was completely inhibited by Cr6+, Ag 2+, Cd2+, Hg2+, Cu2+, Zn 2+ and Pb2+ during an initial screening. When testing at various concentrations, the above heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for Hg2+, Cu 2+, Ag , Cr6+, Cd2+, Pb2+, and Zn2+were 0.071, 0.088, 0.088, 0.87, 0.913, 1.1, and 1.572 mg/L, respectively. The Limit of Detection LOD and Limit of Quantification LOQ values of the heavy metals are within the range of the Maximum Permissibility Limit MPL allowed by the Malaysian Department of Environment DOE for Class-Ill rivers. The IC50 values for these heavy metals are comparable, and some are lower than those of the currently known assays, such as immobilised urease, Daphnia magna, Microtox™, rainbow trout, papain and bromelain assays. Field trial works on an industrial drainage site had shown that the developed assay is applicable in detecting heavy metals in the water. The assay can be carried out in less than 30 min under ambient temperature. Parlar Scientific Publications 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf Aidil, Mohd S. and Sabullah, Mohd Khalidan and Halmi, Mohd Izuan Effendi and Sulaiman, Rosni and Shukor, Mohd Shukri and Abd. Shukor, Mohd. Yunus and Shaharuddin, Noor Azmi and Syed, Mohd Arif and Amir Hamzah, Amir Syahir (2013) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878). Fresenius Environmental Bulletin, 22 (12). pp. 3572-3576. ISSN 1018-4619 |
| spellingShingle | Aidil, Mohd S. Sabullah, Mohd Khalidan Halmi, Mohd Izuan Effendi Sulaiman, Rosni Shukor, Mohd Shukri Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Syed, Mohd Arif Amir Hamzah, Amir Syahir Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title_full | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title_fullStr | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title_full_unstemmed | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title_short | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) |
| title_sort | assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from pangasius hypophthalmus (sauvage, 1878) |
| url | http://psasir.upm.edu.my/id/eprint/28046/ http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf |