The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Mary Ann Liebert
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28045/ http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf |
| _version_ | 1848846006577266688 |
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| author | Ch'ng, Wei Choong Stanbridge, Eric J. Yusoff, Khatijah Shafee, Norazizah |
| author_facet | Ch'ng, Wei Choong Stanbridge, Eric J. Yusoff, Khatijah Shafee, Norazizah |
| author_sort | Ch'ng, Wei Choong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), is active and regulates expression of many genes that contribute to aggressive malignancy, radio-, and chemo-resistance. To investigate the oncolytic efficacy of a highly virulent (velogenic) Newcastle disease virus (NDV) in the presence or absence of HIF-2α, renal cell carcinoma (RCC) cell lines with defective or reconstituted wild-type (wt) von Hippel-Lindau (VHL) activity were used. We show that these RCC cells responded to NDV by producing only interferon (IFN)-β, but not IFN-α, and are associated with increased STAT1 phosphorylation. Restoration of wt VHL expression enhanced NDV-induced IFN-β production, leading to prolonged STAT1 phosphorylation and increased cell death. Hypoxia augmented NDV oncolytic activity regardless of the cells' HIF-2α levels. These results highlight the potential of oncolytic NDV as a potent therapeutic agent in the killing of hypoxic cancer cells. |
| first_indexed | 2025-11-15T08:55:51Z |
| format | Article |
| id | upm-28045 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:51Z |
| publishDate | 2013 |
| publisher | Mary Ann Liebert |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280452016-04-21T07:28:34Z http://psasir.upm.edu.my/id/eprint/28045/ The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling Ch'ng, Wei Choong Stanbridge, Eric J. Yusoff, Khatijah Shafee, Norazizah Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), is active and regulates expression of many genes that contribute to aggressive malignancy, radio-, and chemo-resistance. To investigate the oncolytic efficacy of a highly virulent (velogenic) Newcastle disease virus (NDV) in the presence or absence of HIF-2α, renal cell carcinoma (RCC) cell lines with defective or reconstituted wild-type (wt) von Hippel-Lindau (VHL) activity were used. We show that these RCC cells responded to NDV by producing only interferon (IFN)-β, but not IFN-α, and are associated with increased STAT1 phosphorylation. Restoration of wt VHL expression enhanced NDV-induced IFN-β production, leading to prolonged STAT1 phosphorylation and increased cell death. Hypoxia augmented NDV oncolytic activity regardless of the cells' HIF-2α levels. These results highlight the potential of oncolytic NDV as a potent therapeutic agent in the killing of hypoxic cancer cells. Mary Ann Liebert 2013-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf Ch'ng, Wei Choong and Stanbridge, Eric J. and Yusoff, Khatijah and Shafee, Norazizah (2013) The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling. Journal of Interferon & Cytokine Research, 33 (7). pp. 346-354. ISSN 1079-9907; ESSN: 1557-7465 http://online.liebertpub.com/doi/abs/10.1089/jir.2012.0095 10.1089/jir.2012.0095 |
| spellingShingle | Ch'ng, Wei Choong Stanbridge, Eric J. Yusoff, Khatijah Shafee, Norazizah The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title | The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title_full | The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title_fullStr | The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title_full_unstemmed | The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title_short | The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling |
| title_sort | oncolytic activity of newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von hippel-lindau and interferon-β signaling |
| url | http://psasir.upm.edu.my/id/eprint/28045/ http://psasir.upm.edu.my/id/eprint/28045/ http://psasir.upm.edu.my/id/eprint/28045/ http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf |