In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates

QR Code

In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates

The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus aureus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular dia...

Full description

Bibliographic Details
Main Authors:	Suhaili, Zarizal, Johari, Saiful Azmi, Sajili, Mohammad Hailmi, Yahya, Azifah, Zakaria, Zainul Amiruddin, Mohd Desa, Mohd Nasir, Ali, Abdul Manaf
Format:	Article
Language:	English
Published:	Institute of Research and Development, Walailak University 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/28043/ http://psasir.upm.edu.my/id/eprint/28043/1/28043.pdf

Similar Items

Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA)
by: Atshan, Salman Sahab, et al.
Published: (2013)

Isolation, Identification And Pcr Detection Of Methicillin Resistant Staphylococcus Aureus (Mrsa) In Cow Milk Samples From Perak
by: Anamalai, Sanggari
Published: (2016)

Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA).
by: Saiful, Azmi Johari, et al.
Published: (2008)

Antimicrobial activities of plant extracts against methicillin-susceptible and methicillin-resistant Staphylococcus aureus
by: Chuah, E. L., et al.
Published: (2014)

Methicillin-resistant staphylococcus aureus (MRSA): epidemiology and SCCmec typing
by: Mohamamd Alarosi, Nasreen, et al.
Published: (2017)

In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA)
by: Bitrus, Asinamai Athliamai, et al.
Published: (2017)

PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene
by: Merlino, J., et al.
Published: (2010)

Comparative genomics analysis and molecular characterization of community and hospital-associated methicillin-resistant Staphylococcus aureus
by: Suhaili, Zarizal
Published: (2019)

Development Of A Thermostabilized Multiplex PCR Assay For The Rapid Detection Of Methicillin-Resistant Staphylococcus Aureus
by: Al-Talib, Hassanain I.
Published: (2010)

Evaluation of the conventional test methods for diagnosis of methicillin-resistant staphylococcus aureus (MRSA)
by: Lee Hui, Hua
Published: (2008)

Application of plant metabolites to overcome antibiotic resistance of methicillin resistant Staphylococcus aureus (MRSA)
by: Santiago, Carolina
Published: (2015)

Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon
by: New, Chia Yeung, et al.
Published: (2016)

Antibiotic resistance evolution of Methicillin Resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon
by: New, C. Y., et al.
Published: (2016)

Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
by: Kanagarajah, Rajwin Raja, et al.
Published: (2017)

Analysis of methicillin-resistant staphylococcus aureus (MRSA) and methicillin- susceptible staphylococcus aureus (MSSA) infection in Hospital Pakar Sultanah Fatimah (HPSF) / Nur Hafizah Mohamad Nasir
by: Mohamad Nasir, Nur Hafizah
Published: (2014)

Molecular characterization of nosocomial methicillin resistant staphylococci aureus by Rep-PCR.
by: Amin Nordin, Syafinaz, et al.
Published: (2011)

Differentiation of Staphylococcus aureus and Staphylococcus epidermidis by PCR for the fibrinogen binding protein gene.
by: Babra, Charlene, et al.
Published: (2013)

Inhibitory and resistance-modifying potential of plant-based alkaloids against methicillin-resistant staphylococcus aureus (MRSA)
by: Mohtar, Mastura, et al.
Published: (2009)

Molecular epidemiology: a valuable tool for determination of emerging and clonality of methicillin resistant Staphylococcus aureus (MRSA)
by: Bitrus, Asinamai Athliamai, et al.
Published: (2016)

Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pet animals and pet and non-pet owners
by: Abdul Aziz, Saleha, et al.
Published: (2006)

Influence of biofilm-forming lactic acid bacteria against methicillin-resistant Staphylococcus aureus (MRSA S547)
by: M.Kumar, Laavanya, et al.
Published: (2017)

Colonization of Methicillin-Resistant Staphylococcus aureus (MRSA) among medical students in tertiary institution in Central Malaysia
by: Lekhraj Rampal, Sanjiv Rampal, et al.
Published: (2020)

Synergistic Activity Of Ceftriaxone And Polyalthia Longifolia Leaves Extract Against Methicillin Resistant Staphylococcus Aureus (Mrsa)
by: Valiappan, Ranjutha
Published: (2021)

Stability, toxicity, and antibacterial potential of Gallic Acid-Loaded Graphene Oxide (gago) against methicillin-resistant Staphylococcus Aureus (MRSA) strains
by: Shamsi, Suhaili, et al.
Published: (2022)

Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) Infection Among Patients in Surgical ad Orthopaedic Wards of PPUKM
by: Mohamad Noor, Nurul Nadiah, et al.
Published: (2015)

Codonopsinine derivatives as potential inhibitory agents against methicillin- resistant staphylococcus aureus (MRSA) / Saiful Azmi Johari
by: Johari, Saiful Azmi
Published: (2018)

Membrane biosynthesis gene disruption in methicillin- resistant Staphylococcus aureus (MRSA) as potential mechanism for reducing antibiotic resistance
by: Mohamed Alipiah, Norfarrah, et al.
Published: (2015)

Proteomic analysis reveals phage-driven metabolic shifts and biofilm disruption in methicillin-resistant Staphylococcus aureus (MRSA)
by: Dakheel, Khulood Hamid, et al.
Published: (2025)

Comparison of a multiplexed massARRAY system with real-time allele-specific PCR technology for genotyping of methicillin-resistant staphylococcus aureus
by: Syrmis, M., et al.
Published: (2011)

Demonstration of antigenic and specific whole cell proteins for methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
by: Ismail, Noraini
Published: (2002)

Knowing prior methicillin-resistant Staphylococcus aureus (MRSA) infection or colonization status increases the empirical use of glycopeptides in MRSA bacteraemia and may decrease mortality.
by: Phillips, M., et al.
Published: (2013)

Not Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA)! A Clinician’s Guide to Community MRSA - Its Evolving Antimicrobial Resistance and Implications for Therapy
by: Chua, K., et al.
Published: (2011)

Emergence and molecular characterization of clonal complex 398 (CC398) methicillin-resistant Staphylococcus aureus (MRSA) in New Zealand.
by: Williamson, D., et al.
Published: (2013)

Methicillin resistant Staphylococcus aureus (MRSA): an emerging veterinary and zoonotic pathogen of public health concern and some studies in Malaysia.
by: Abdul Aziz, Saleha, et al.
Published: (2010)

Persistence of antibacterial resistance and virulence gene profile of methicillin resistant Staphylococcus aureus (MRSA) isolated from humans and animals
by: Bitrus, Asinamai Athliamai, et al.
Published: (2016)

Development Of Fluorescing Methicillin Resistant Staphylococcus Aureus (Mrsa) As A Tool For The Screening Of Antibacterial Properties Of Mimusops Elengi Linn
by: Zaabar, Wan Razlin Wan
Published: (2011)

Intrafamilial transmission of methicillin-resistant Staphylococcus aureus
by: Pozzi Langhi, S., et al.
Published: (2009)

Methicillin resistant Staphylococcus aureus on horses wound
by: Ahmad Nadzir, Nur Adilah, et al.
Published: (2015)

In vitro activities of 28 antimicrobial agents against methicillin-resistant staphylococcus aureus (MRSA) from a clinical setting in Malaysia.
by: Neela, Vasantha Kumari, et al.
Published: (2008)

The impact of Methicillin-Resistant Staphylococcus aureus (MRSA) evolution on clinical, phenotypic and genotypic characteristics of MRSA strains isolated in a tertiary teaching hospital / Sit Pik San
by: Sit, Pik San
Published: (2018)