Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen α2 (I) chain (pAb1...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28033/ http://psasir.upm.edu.my/id/eprint/28033/1/Determination%20of%20porcine%20gelatin%20in%20edible%20bird%27s%20nest%20by%20competitive%20indirect%20ELISA%20based%20on%20anti-peptide%20polyclonal%20antibody.pdf |
| _version_ | 1848846003224969216 |
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| author | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_facet | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_sort | Tukiran, Nur Azira |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen α2 (I) chain (pAb1 and pAb2) and α1 (I) chain (pAb3). The limit of detection (IC15) of the three ELISAs was 0.033, 0.082 and 0.052 μg/mL respectively. The median inhibitory concentration (IC50) of pAb1, pAb2 and pAb3 was 0.265, 0.394 and 0.228 μg/mL respectively, as well as able to recognise porcine and bovine gelatins. pAb1 showed slight cross-reactivity with cave nest and egg white, while pAb2 exhibited slight cross-reactivity with blood cave nest and egg white. No cross-reactivity was observed with EBNs and egg white for pAb3. The recoveries of porcine gelatin spiked EBNs were in the range of 62.8–125.4% with intra- and inter-day coefficient of variants (CVs) of 2.9–5.4% and 4.7–9.6% respectively when using pAb3. Taking into account all abovementioned factors, pAb3 appeared sufficient for EBN authentication. |
| first_indexed | 2025-11-15T08:55:48Z |
| format | Article |
| id | upm-28033 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:48Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280332016-06-20T03:05:05Z http://psasir.upm.edu.my/id/eprint/28033/ Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen α2 (I) chain (pAb1 and pAb2) and α1 (I) chain (pAb3). The limit of detection (IC15) of the three ELISAs was 0.033, 0.082 and 0.052 μg/mL respectively. The median inhibitory concentration (IC50) of pAb1, pAb2 and pAb3 was 0.265, 0.394 and 0.228 μg/mL respectively, as well as able to recognise porcine and bovine gelatins. pAb1 showed slight cross-reactivity with cave nest and egg white, while pAb2 exhibited slight cross-reactivity with blood cave nest and egg white. No cross-reactivity was observed with EBNs and egg white for pAb3. The recoveries of porcine gelatin spiked EBNs were in the range of 62.8–125.4% with intra- and inter-day coefficient of variants (CVs) of 2.9–5.4% and 4.7–9.6% respectively when using pAb3. Taking into account all abovementioned factors, pAb3 appeared sufficient for EBN authentication. Elsevier 2016-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28033/1/Determination%20of%20porcine%20gelatin%20in%20edible%20bird%27s%20nest%20by%20competitive%20indirect%20ELISA%20based%20on%20anti-peptide%20polyclonal%20antibody.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2016) Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody. Food Control, 59. pp. 561-566. ISSN 0956-7135; ESSN: 1873-7129 http://www.sciencedirect.com/science/article/pii/S0956713515300682 10.1016/j.foodcont.2015.06.039 |
| spellingShingle | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title | Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title_full | Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title_fullStr | Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title_full_unstemmed | Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title_short | Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody |
| title_sort | determination of porcine gelatin in edible bird's nest by competitive indirect elisa based on anti-peptide polyclonal antibody |
| url | http://psasir.upm.edu.my/id/eprint/28033/ http://psasir.upm.edu.my/id/eprint/28033/ http://psasir.upm.edu.my/id/eprint/28033/ http://psasir.upm.edu.my/id/eprint/28033/1/Determination%20of%20porcine%20gelatin%20in%20edible%20bird%27s%20nest%20by%20competitive%20indirect%20ELISA%20based%20on%20anti-peptide%20polyclonal%20antibody.pdf |