Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM
Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time:...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/28020/ http://psasir.upm.edu.my/id/eprint/28020/1/28020.pdf |
| _version_ | 1848845999417589760 |
|---|---|
| author | Basri, Mahiran Kassim, Mohd Azlan Mohamad, Rosfarizan Ariff, Arbakariya |
| author_facet | Basri, Mahiran Kassim, Mohd Azlan Mohamad, Rosfarizan Ariff, Arbakariya |
| author_sort | Basri, Mahiran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Lipozyme TL IM could be used for 5 cycles with conversion above 50%. The alcoholysis reaction kinetic follows the Ping-Pong Bi-Bi mechanism characterized by the Vmax, Km(PO), and Km(OA) values of 32.7 mmol/min, 0.3147 mmol/ml and 0.9483 mmol/ml, respectively. The relationship between initial reaction rate and temperature was also established based on the Arrhenius law. |
| first_indexed | 2025-11-15T08:55:44Z |
| format | Article |
| id | upm-28020 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:44Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280202016-03-07T06:46:53Z http://psasir.upm.edu.my/id/eprint/28020/ Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM Basri, Mahiran Kassim, Mohd Azlan Mohamad, Rosfarizan Ariff, Arbakariya Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Lipozyme TL IM could be used for 5 cycles with conversion above 50%. The alcoholysis reaction kinetic follows the Ping-Pong Bi-Bi mechanism characterized by the Vmax, Km(PO), and Km(OA) values of 32.7 mmol/min, 0.3147 mmol/ml and 0.9483 mmol/ml, respectively. The relationship between initial reaction rate and temperature was also established based on the Arrhenius law. Elsevier 2013-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28020/1/28020.pdf Basri, Mahiran and Kassim, Mohd Azlan and Mohamad, Rosfarizan and Ariff, Arbakariya (2013) Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM. Journal of Molecular Catalysis B: Enzymatic, 85-86. pp. 214-219. ISSN 1381-1177; ESSN: 1873-3158 10.1016/j.molcatb.2012.09.013 |
| spellingShingle | Basri, Mahiran Kassim, Mohd Azlan Mohamad, Rosfarizan Ariff, Arbakariya Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title | Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title_full | Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title_fullStr | Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title_full_unstemmed | Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title_short | Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
| title_sort | optimization and kinetic study on the synthesis of palm oil ester using lipozyme tl im |
| url | http://psasir.upm.edu.my/id/eprint/28020/ http://psasir.upm.edu.my/id/eprint/28020/ http://psasir.upm.edu.my/id/eprint/28020/1/28020.pdf |