Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility
The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28009/ http://psasir.upm.edu.my/id/eprint/28009/1/Overexpression%20of%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20TAPETUM%20DEVELOPMENT1-like%20Eg707%20in%20rice%20affects%20cell%20division%20and%20differentiation%20and%20reduces%20fertility.pdf |
| _version_ | 1848845996360990720 |
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| author | Thuc, Le Vinh Geelen, Danny Ky, Huynh Ooi, Siew Eng Napis, Suhaimi Sinniah, Uma Rani Namasivayam, Parameswari |
| author_facet | Thuc, Le Vinh Geelen, Danny Ky, Huynh Ooi, Siew Eng Napis, Suhaimi Sinniah, Uma Rani Namasivayam, Parameswari |
| author_sort | Thuc, Le Vinh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared to pale wild type and negative control calli. Regenerated transgenic rice plants exhibited a reduction in organ size and plant height, rolled, erect leaves, less tillers, increased chlorophyll content, and reduced fertility with smaller green seeds. At the molecular level Eg707 overexpression caused an increase in the transcription of SAPK9, a SnRK2 protein kinase family member that is activated by ABA and hyperosmotic stress. Together, the results show that ectopic Eg707 expression influences cell division and differentiation, presumably via altered hormone homeostasis. |
| first_indexed | 2025-11-15T08:55:41Z |
| format | Article |
| id | upm-28009 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:41Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280092016-03-07T05:12:31Z http://psasir.upm.edu.my/id/eprint/28009/ Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility Thuc, Le Vinh Geelen, Danny Ky, Huynh Ooi, Siew Eng Napis, Suhaimi Sinniah, Uma Rani Namasivayam, Parameswari The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared to pale wild type and negative control calli. Regenerated transgenic rice plants exhibited a reduction in organ size and plant height, rolled, erect leaves, less tillers, increased chlorophyll content, and reduced fertility with smaller green seeds. At the molecular level Eg707 overexpression caused an increase in the transcription of SAPK9, a SnRK2 protein kinase family member that is activated by ABA and hyperosmotic stress. Together, the results show that ectopic Eg707 expression influences cell division and differentiation, presumably via altered hormone homeostasis. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28009/1/Overexpression%20of%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20TAPETUM%20DEVELOPMENT1-like%20Eg707%20in%20rice%20affects%20cell%20division%20and%20differentiation%20and%20reduces%20fertility.pdf Thuc, Le Vinh and Geelen, Danny and Ky, Huynh and Ooi, Siew Eng and Napis, Suhaimi and Sinniah, Uma Rani and Namasivayam, Parameswari (2013) Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility. Molecular Biology Reports, 40 (2). pp. 1579-1590. ISSN 0301-4851; ESSN: 1573-4978 http://link.springer.com/article/10.1007%2Fs11033-012-2206-7 10.1007/s11033-012-2206-7 |
| spellingShingle | Thuc, Le Vinh Geelen, Danny Ky, Huynh Ooi, Siew Eng Napis, Suhaimi Sinniah, Uma Rani Namasivayam, Parameswari Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title | Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title_full | Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title_fullStr | Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title_full_unstemmed | Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title_short | Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility |
| title_sort | overexpression of the oil palm (elaeis guineensis jacq.) tapetum development1-like eg707 in rice affects cell division and differentiation and reduces fertility |
| url | http://psasir.upm.edu.my/id/eprint/28009/ http://psasir.upm.edu.my/id/eprint/28009/ http://psasir.upm.edu.my/id/eprint/28009/ http://psasir.upm.edu.my/id/eprint/28009/1/Overexpression%20of%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20TAPETUM%20DEVELOPMENT1-like%20Eg707%20in%20rice%20affects%20cell%20division%20and%20differentiation%20and%20reduces%20fertility.pdf |