Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene
A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28007/ http://psasir.upm.edu.my/id/eprint/28007/1/28007.pdf |
| _version_ | 1848845995849285632 |
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| author | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom |
| author_facet | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom |
| author_sort | Sekeli, Rogayah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tumefaciens-mediated transformation method. Regenerated transformed shoots, each measuring approximately 3-4 cm in height, were cultured in liquid half-strength Murashige and Skoog (MS) medium or sterile distilled water, and with either perlite or vermiculite supplementation. All the culturing processes were conducted either under sterile or nonsterile condition. The results showed that rooting under sterile condition was better. Shoots cultured in half-strength MS medium supplemented with vermiculite exhibited a 92.5% rooting efficiency while perlite showed 77.5%. The survival rate of the vermiculite-grown transformed papaya plantlets after transfer into soil, contained in polybags, was 94%, and the rate after transfer into the ground was 92%. Morpho-histological analyses revealed that the tap roots were more compact, which might have contributed to the high survival rates of the plantlets. |
| first_indexed | 2025-11-15T08:55:41Z |
| format | Article |
| id | upm-28007 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:41Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280072017-11-10T05:34:50Z http://psasir.upm.edu.my/id/eprint/28007/ Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tumefaciens-mediated transformation method. Regenerated transformed shoots, each measuring approximately 3-4 cm in height, were cultured in liquid half-strength Murashige and Skoog (MS) medium or sterile distilled water, and with either perlite or vermiculite supplementation. All the culturing processes were conducted either under sterile or nonsterile condition. The results showed that rooting under sterile condition was better. Shoots cultured in half-strength MS medium supplemented with vermiculite exhibited a 92.5% rooting efficiency while perlite showed 77.5%. The survival rate of the vermiculite-grown transformed papaya plantlets after transfer into soil, contained in polybags, was 94%, and the rate after transfer into the ground was 92%. Morpho-histological analyses revealed that the tap roots were more compact, which might have contributed to the high survival rates of the plantlets. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28007/1/28007.pdf Sekeli, Rogayah and Abdullah, Janna Ong and Namasivayam, Parameswari and Muda, Pauziah and Abu Bakar, Umi Kalsom (2013) Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene. ISRN Biotechnology, 2013. art. no. 958945. pp. 1-10. ISSN 2090-9403 http://www.hindawi.com/journals/isrn/2013/958945/abs/ 10.5402/2013/958945 |
| spellingShingle | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title | Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title_full | Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title_fullStr | Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title_full_unstemmed | Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title_short | Better rooting procedure to enhance survival rate of field grown Malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| title_sort | better rooting procedure to enhance survival rate of field grown malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene |
| url | http://psasir.upm.edu.my/id/eprint/28007/ http://psasir.upm.edu.my/id/eprint/28007/ http://psasir.upm.edu.my/id/eprint/28007/ http://psasir.upm.edu.my/id/eprint/28007/1/28007.pdf |