Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis)
The AP2/ERF (APETALA2/ethylene-responsive factor) proteins play important regulatory roles in plant response to stress as well as plant growth and developmental processes. Using yeast one-hybrid system and three tandem repeats of the ERE (ethylene-responsive element) as a probe, an ethylene-responsi...
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| Format: | Article |
| Language: | English |
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Southern Cross Publishing
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28006/ http://psasir.upm.edu.my/id/eprint/28006/1/Isolation%20and%20characterization%20of%20an%20ethylene-responsive%20element%20binding%20protein%20%28EgEREBP%29%20from%20oil%20palm%20%28Elaeis%20guineensis%29.pdf |
| _version_ | 1848845995556732928 |
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| author | Omidvar, Vahid Abdullah, Siti Nor Akmar Ho, Chai Ling Mahmood, Maziah |
| author_facet | Omidvar, Vahid Abdullah, Siti Nor Akmar Ho, Chai Ling Mahmood, Maziah |
| author_sort | Omidvar, Vahid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The AP2/ERF (APETALA2/ethylene-responsive factor) proteins play important regulatory roles in plant response to stress as well as plant growth and developmental processes. Using yeast one-hybrid system and three tandem repeats of the ERE (ethylene-responsive element) as a probe, an ethylene-responsive cDNA clone designated as EgEREBP (Elaeis guineensis ERE-binding protein) was isolated from oil palm fruits treated with ethylene hormone. The EgEREBP-encoded protein has a conserved AP2/EREBP DNA-binding domain (DNA-BD), a potential nuclear localization sequence, and a putative activation domain. EgEREBP protein was classified as a member of DREB/CBF(dehydration-responsive element binding protein/c-repeat binding factor) proteins based on the multiple sequence alignment and phylogenetic analysis. EgEREBP showed ERE -binding, transcriptional activation, and transactivation properties in yeast and in vitro. Electrophoretic mobility shift assay (EMSA) revealed that EgEREBP could bind to the DRE in addition to the ERE sequence. RT-PCR analysis revealed that the expression of EgEREBP gene was induced in oil palm fruits in response to a range of hormone treatments, includingabscisic acid (ABA), methyl jasmonate (MJ), salicylic acid (SA) and abiotic stresses,including drought, cold and high-salinity. Our data demonstrated the regulatory functions of EgEREBP gene in stress response and ethylene signaling in oil palm which could be potentially useful for the production of stress-tolerant transgenic crops. |
| first_indexed | 2025-11-15T08:55:40Z |
| format | Article |
| id | upm-28006 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:40Z |
| publishDate | 2013 |
| publisher | Southern Cross Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280062016-03-07T04:51:05Z http://psasir.upm.edu.my/id/eprint/28006/ Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) Omidvar, Vahid Abdullah, Siti Nor Akmar Ho, Chai Ling Mahmood, Maziah The AP2/ERF (APETALA2/ethylene-responsive factor) proteins play important regulatory roles in plant response to stress as well as plant growth and developmental processes. Using yeast one-hybrid system and three tandem repeats of the ERE (ethylene-responsive element) as a probe, an ethylene-responsive cDNA clone designated as EgEREBP (Elaeis guineensis ERE-binding protein) was isolated from oil palm fruits treated with ethylene hormone. The EgEREBP-encoded protein has a conserved AP2/EREBP DNA-binding domain (DNA-BD), a potential nuclear localization sequence, and a putative activation domain. EgEREBP protein was classified as a member of DREB/CBF(dehydration-responsive element binding protein/c-repeat binding factor) proteins based on the multiple sequence alignment and phylogenetic analysis. EgEREBP showed ERE -binding, transcriptional activation, and transactivation properties in yeast and in vitro. Electrophoretic mobility shift assay (EMSA) revealed that EgEREBP could bind to the DRE in addition to the ERE sequence. RT-PCR analysis revealed that the expression of EgEREBP gene was induced in oil palm fruits in response to a range of hormone treatments, includingabscisic acid (ABA), methyl jasmonate (MJ), salicylic acid (SA) and abiotic stresses,including drought, cold and high-salinity. Our data demonstrated the regulatory functions of EgEREBP gene in stress response and ethylene signaling in oil palm which could be potentially useful for the production of stress-tolerant transgenic crops. Southern Cross Publishing 2013-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28006/1/Isolation%20and%20characterization%20of%20an%20ethylene-responsive%20element%20binding%20protein%20%28EgEREBP%29%20from%20oil%20palm%20%28Elaeis%20guineensis%29.pdf Omidvar, Vahid and Abdullah, Siti Nor Akmar and Ho, Chai Ling and Mahmood, Maziah (2013) Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis). Australian Journal of Crop Science, 7 (2). pp. 219-226. ISSN 1835-2693; ESSN: 1835-2707 http://www.cropj.com/february2013.html |
| spellingShingle | Omidvar, Vahid Abdullah, Siti Nor Akmar Ho, Chai Ling Mahmood, Maziah Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title | Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title_full | Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title_fullStr | Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title_full_unstemmed | Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title_short | Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis) |
| title_sort | isolation and characterization of an ethylene-responsive element binding protein (egerebp) from oil palm (elaeis guineensis) |
| url | http://psasir.upm.edu.my/id/eprint/28006/ http://psasir.upm.edu.my/id/eprint/28006/ http://psasir.upm.edu.my/id/eprint/28006/1/Isolation%20and%20characterization%20of%20an%20ethylene-responsive%20element%20binding%20protein%20%28EgEREBP%29%20from%20oil%20palm%20%28Elaeis%20guineensis%29.pdf |