| Summary: | Legumes play an important role in providing nitrogen in ecological system. Numerous
symbiotic and non-symbiotic endophytic bacteria have been isolated from root nodules of many types of legume plants (Zakhia et al. 2006: Kan et al. 2007: Palaniappan et al.
2010). Rhizobia-legume is an example of symbiotic endophylcs which are able to supply
ammonia to the legume plants by fixing nitrogen in the atmosphere.
As well as the rhizobia, some non-symbiotic endophytes have been found inside root
nodules and they do not visibly harm the host plant. Therefore, these endophytes get
benefits for themselves and at the same time they have the protection from harsh
environment and microbial competition by the host plant. They were mainly Bacillus,
Pseudomonas and Enterobacteria species (Zakhia et al. 2006; Kan et al 2007). Some
endophytic bacteria that have beneficial effects on plants are referred to as PGPB. PGPB
can promote plant growth such as by diazotrophs (nitrogen fixing microorganism) to fix
nitrogen, solubilization of phosphate, and hormonal plant growth promoters. Thus. the
objective of this study was to identify and isolated symbiotic and non-symbiotic
endophytic bacteria from soybean and winged bean by 16S rDNA that have the ability in
nitrogen fixation, phosphate solubilization and also capable as producers of plant growth
honnone IAA.
|