A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent

The bacterial strain EB 172, isolated from digester treating palm oil mill effluent, was investigated by polyphasic taxanomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses usnign the 16S rRNA gene sequence showed that...

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Main Authors: Hassan, Mohd Ali, Zakaria @ Mamat, Mohd Rafein, Abd. Aziz, Suriani, Mohamad Ghazali, Farinazleen, Shirai, Yoshihito, Wakisaka, Minato
Format: Patent
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/27636/
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author Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Abd. Aziz, Suriani
Mohamad Ghazali, Farinazleen
Shirai, Yoshihito
Wakisaka, Minato
author_facet Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Abd. Aziz, Suriani
Mohamad Ghazali, Farinazleen
Shirai, Yoshihito
Wakisaka, Minato
author_sort Hassan, Mohd Ali
building UPM Institutional Repository
collection Online Access
description The bacterial strain EB 172, isolated from digester treating palm oil mill effluent, was investigated by polyphasic taxanomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses usnign the 16S rRNA gene sequence showed that the strains C. terrigena (96.8%), C. koreensis (93.4%), C. composti (92.9%), and C.kerstersii (91.1%). The ability of C. putranesis to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium is unique in Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics. The DNA G+C content of the genomic DNA was 59.1 mol%. It is evident from the genotypic and phenotypic data that strain Comamonas putranesis represents a novel species in the genus Comamonas, for which the name Comamonas putranesis sp. nov. is proposed.
first_indexed 2025-11-15T08:54:07Z
format Patent
id upm-27636
institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T08:54:07Z
publishDate 2009
recordtype eprints
repository_type Digital Repository
spelling upm-276362016-09-08T02:47:38Z http://psasir.upm.edu.my/id/eprint/27636/ A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Abd. Aziz, Suriani Mohamad Ghazali, Farinazleen Shirai, Yoshihito Wakisaka, Minato The bacterial strain EB 172, isolated from digester treating palm oil mill effluent, was investigated by polyphasic taxanomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses usnign the 16S rRNA gene sequence showed that the strains C. terrigena (96.8%), C. koreensis (93.4%), C. composti (92.9%), and C.kerstersii (91.1%). The ability of C. putranesis to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium is unique in Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics. The DNA G+C content of the genomic DNA was 59.1 mol%. It is evident from the genotypic and phenotypic data that strain Comamonas putranesis represents a novel species in the genus Comamonas, for which the name Comamonas putranesis sp. nov. is proposed. 2009-06-29 Patent NonPeerReviewed Mohd Ali Hassan (2009) A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent. UNSPECIFIED.
spellingShingle Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Abd. Aziz, Suriani
Mohamad Ghazali, Farinazleen
Shirai, Yoshihito
Wakisaka, Minato
A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title_full A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title_fullStr A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title_full_unstemmed A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title_short A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
title_sort novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent
url http://psasir.upm.edu.my/id/eprint/27636/