Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isola...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/27407/ http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf |
| _version_ | 1848845837451395072 |
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| author | Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor |
| author_facet | Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor |
| author_sort | Tasrip, Nor Asmara |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isolates were subjected to antibacterial assay against 2 pathogenic bacteria of gram positive and gram negative (Escherichia coli ATCC 25922 & Staphylococcus aureus ATCC 25923), by perpendicular streak method using MHA and TSA as the test media. With MHA as the test media in the antibacterial assay, > 50 % of the isolates showed antibacterial activity to at least one of the test bacteria as compared by using TSA as the test media. Color series of aerial mycelium was described based on growth on ISP-2 medium; there was a significant association between grey and non-grey color series of the aerial mycelium in
association with the antibacterial activity of the isolates on MHA (89% vs. 42% respectively, P = 0.013) and TSA (78% vs. 35% respectively, P = 0.025). As a conclusion, different chemical composition of the test media could have an effect on the outcome of the antibacterial assay (MHA vs. TSA). The observed relationship between aerial mass-color (grey vs. nongrey) and antibacterial activity warrant further investigation in a larger sample of isolates. |
| first_indexed | 2025-11-15T08:53:10Z |
| format | Conference or Workshop Item |
| id | upm-27407 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:53:10Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-274072018-04-24T04:04:22Z http://psasir.upm.edu.my/id/eprint/27407/ Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor 40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isolates were subjected to antibacterial assay against 2 pathogenic bacteria of gram positive and gram negative (Escherichia coli ATCC 25922 & Staphylococcus aureus ATCC 25923), by perpendicular streak method using MHA and TSA as the test media. With MHA as the test media in the antibacterial assay, > 50 % of the isolates showed antibacterial activity to at least one of the test bacteria as compared by using TSA as the test media. Color series of aerial mycelium was described based on growth on ISP-2 medium; there was a significant association between grey and non-grey color series of the aerial mycelium in association with the antibacterial activity of the isolates on MHA (89% vs. 42% respectively, P = 0.013) and TSA (78% vs. 35% respectively, P = 0.025). As a conclusion, different chemical composition of the test media could have an effect on the outcome of the antibacterial assay (MHA vs. TSA). The observed relationship between aerial mass-color (grey vs. nongrey) and antibacterial activity warrant further investigation in a larger sample of isolates. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf Tasrip, Nor Asmara and Mohd Desa, Mohd Nasir and Cheah, Yoke Kqueen and Chong, Pei Pei and Shamsudin, Mariana Nor (2012) Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis. In: 4th International Conference on the Development of Biomedical Engineering, 8-12 Jan. 2012, Ho Chi Minh, Vietnam. (pp. 135-136). 10.1007/978-3-642-32183-2_35 |
| spellingShingle | Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title | Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title_full | Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title_fullStr | Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title_full_unstemmed | Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title_short | Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis |
| title_sort | associations between cultural characteristics and antibacterial activities of streptomyces isolates: a preliminary analysis |
| url | http://psasir.upm.edu.my/id/eprint/27407/ http://psasir.upm.edu.my/id/eprint/27407/ http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf |