Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1

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Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...

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Bibliographic Details
Main Authors:	Ruslan, Rudzanna, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Mohamad Ali, Mohd Shukuri, Basri, Mahiran, Salleh, Abu Bakar
Format:	Article
Language:	English
Published:	MDPI AG 2012
Online Access:	http://psasir.upm.edu.my/id/eprint/27295/ http://psasir.upm.edu.my/id/eprint/27295/1/27295.pdf

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