Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines
Zinc oxide nanoparticles (ZnO-NPs) are among the most appropriate metal oxide nanoparticles to exhibit significant potential for treatment properties in a broad spectrum of applications in biomedicine, such as in the treatment of various cancers. The aim of this study was to evaluate the in vitro cy...
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| Format: | Conference or Workshop Item |
| Language: | English |
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UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/27062/ http://psasir.upm.edu.my/id/eprint/27062/1/ABSTRACT%20CAC%202014_2%20medic%2018.pdf |
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| author | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Azizi, Susan Md. Tahir, Paridah Chartrand, Max Stanley Yeap, Swee Keong |
| author_facet | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Azizi, Susan Md. Tahir, Paridah Chartrand, Max Stanley Yeap, Swee Keong |
| author_sort | Namvar, Farideh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Zinc oxide nanoparticles (ZnO-NPs) are among the most appropriate metal oxide nanoparticles to exhibit significant potential for treatment properties in a broad spectrum of applications in biomedicine, such as in the treatment of various cancers. The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of previously prepared ZnO-NPs using brown seaweed (Sargassum muticum) aqueous extract. Consequently, In vitro anticancer activity was demonstrated in murine cancer cell lines of breast cancer (4T1), lung adenocarcinoma (CRL-1451), colon cancer (CT-26), and acute myelocytic leukemia (WEHI-3). Treated cancer cells with ZnO-NPs for 72 hours demonstrated various levels of cytotoxicity based on calculated IC50 values using MTT assay as follows: 21.7 ± 1.3 μg /mL (4T1), 17.45 ± 1.1 μg /mL (CRL-1451), 11.75 ± 0.8 μg /mL (CT-26) and 5.6 ± 0.55 μg /mL (WEHI-3), respectively. On the other hand, ZnO-NPs treatments for 72 hours showed no toxicity against normal mouse fibroblast (3T3) cell lines. Furthermore, distinct morphological changes were found by utilizing flourescent dyes, as apoptotic population were increased via flowcytometry, while cell cycle block and stimulation of apoptotic proteins were also observed. Additionally, the present study showed that the caspase activations contributed to ZnO-NPs triggered apoptotic death in WEHI-3 cells. Thus, the nature of biosynthesis and the therapeutic potential of ZnO-NPs could prepare the way for further research on the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer. |
| first_indexed | 2025-11-15T08:51:41Z |
| format | Conference or Workshop Item |
| id | upm-27062 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:51:41Z |
| publishDate | 2014 |
| publisher | UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-270622017-10-25T03:04:53Z http://psasir.upm.edu.my/id/eprint/27062/ Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Azizi, Susan Md. Tahir, Paridah Chartrand, Max Stanley Yeap, Swee Keong Zinc oxide nanoparticles (ZnO-NPs) are among the most appropriate metal oxide nanoparticles to exhibit significant potential for treatment properties in a broad spectrum of applications in biomedicine, such as in the treatment of various cancers. The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of previously prepared ZnO-NPs using brown seaweed (Sargassum muticum) aqueous extract. Consequently, In vitro anticancer activity was demonstrated in murine cancer cell lines of breast cancer (4T1), lung adenocarcinoma (CRL-1451), colon cancer (CT-26), and acute myelocytic leukemia (WEHI-3). Treated cancer cells with ZnO-NPs for 72 hours demonstrated various levels of cytotoxicity based on calculated IC50 values using MTT assay as follows: 21.7 ± 1.3 μg /mL (4T1), 17.45 ± 1.1 μg /mL (CRL-1451), 11.75 ± 0.8 μg /mL (CT-26) and 5.6 ± 0.55 μg /mL (WEHI-3), respectively. On the other hand, ZnO-NPs treatments for 72 hours showed no toxicity against normal mouse fibroblast (3T3) cell lines. Furthermore, distinct morphological changes were found by utilizing flourescent dyes, as apoptotic population were increased via flowcytometry, while cell cycle block and stimulation of apoptotic proteins were also observed. Additionally, the present study showed that the caspase activations contributed to ZnO-NPs triggered apoptotic death in WEHI-3 cells. Thus, the nature of biosynthesis and the therapeutic potential of ZnO-NPs could prepare the way for further research on the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer. UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience 2014 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27062/1/ABSTRACT%20CAC%202014_2%20medic%2018.pdf Namvar, Farideh and Rahman, Heshu Sulaiman and Mohamad, Rosfarizan and Azizi, Susan and Md. Tahir, Paridah and Chartrand, Max Stanley and Yeap, Swee Keong (2014) Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines. In: Scientific Cancer Research Poster Competition in conjunction with Cancer Awareness Carnival 2014, 10 May 2014, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. . |
| spellingShingle | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Azizi, Susan Md. Tahir, Paridah Chartrand, Max Stanley Yeap, Swee Keong Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title | Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title_full | Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title_fullStr | Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title_full_unstemmed | Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title_short | Cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| title_sort | cytotoxic effects of bio-synthesized zinc oxide nanoparticles on murine cell lines |
| url | http://psasir.upm.edu.my/id/eprint/27062/ http://psasir.upm.edu.my/id/eprint/27062/1/ABSTRACT%20CAC%202014_2%20medic%2018.pdf |