Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
In this present study, the stem bark of Garcinia maingayi and Garcinia parvifolia were investigated and resulted in the isolation of nine compounds. There are no previous reports on chemical components and biological activities from Garcinia maingayi. The structures of these compounds were elucid...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2005
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/27/ http://psasir.upm.edu.my/id/eprint/27/1/1000548961_t_FS_2005_1.pdf |
| _version_ | 1848838675539951616 |
|---|---|
| author | Lin, Cheow Yuen |
| author_facet | Lin, Cheow Yuen |
| author_sort | Lin, Cheow Yuen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this present study, the stem bark of Garcinia maingayi and Garcinia parvifolia were
investigated and resulted in the isolation of nine compounds. There are no previous
reports on chemical components and biological activities from Garcinia maingayi. The
structures of these compounds were elucidated by using spectroscopic experiments
namely NMR, MS, IR and UV.
Being the first report on Garcinia maingayi, detailed chemical studies have afforded
two triterpenoids, stigmasterol and sitosterol, two xanthones, 1,3,7-trihydroxy-2-(3-
methylbut-2-enyl)-xanthone and 1,3,6,8-tetrahydroxyxanthone, one benzophenone,
isoxanthochymol, and one benzoic acid derivative methyl 3,4-dihydroxybenzoate. The
findings are significant as it contributes to the knowledge of the chemotaxonomy on
Garcinia species and all these compounds are new to the species.
iii
Meanwhile investigations on Garcinia parvifolia have afforded one triterpenoid, α-
amyrin and two xanthones, cowanin and rubraxanthone. Acetylation reaction was
carried out on rubraxanthone to yield triacetate rubraxanthone.
This is also the first report on cytotoxic and larvicidal activities of Garcinia maingayi,
Garcinia parvifolia and rubraxanthone. Cytotoxic tests were performed using HL-60
and CEM-SS cell lines. The crude hexane and chloroform extracts of Garcinia
maingayi were active against HL-60 cell line with IC50 values of less than 30 μg/ml. The
crude hexane and acetone extracts of Garcinia parvifolia were found to be active
against CEM-SS cell line with IC50 values of less than 30 μg/ml meanwhile, the crude
chloroform extract gave a significant activity with an IC50 value of 6.5 μg/ml.
The antimicrobial assay was carried out against four pathogenic bacteria, Methicillin
resistant Staphylococcus aures, Pseudomonas aeruginosa, Staphylococcus typhimurium
and Bacillus subtilis. Most of the crude extracts tested against these microbes gave only
moderate or weak activity. The antifungal activity testing of the plant extracts were
carried out against the fungi Candida albican, Aspergillus ochraceaus, Sacchoromyces
cerevisiae and Candida lypolytica. No activity was observed for all the crude extracts
The larvicidal test was carried out towards the larvae of Aedes aegypti. All the crude
extracts of Garcinia maingayi were weakly active against the larvae with LC50 values of
more than 150 μg/ml. The crude extracts of Garcinia parvifolia showed moderate
activities against the larvae by giving LC50 values of less than 100 μg/ml. The pure
iv
rubraxanthone showed a strong activity against the larvae with a LC50 value of 15.49
μg/ml. |
| first_indexed | 2025-11-15T06:59:19Z |
| format | Thesis |
| id | upm-27 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:59:19Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-272013-05-27T06:45:06Z http://psasir.upm.edu.my/id/eprint/27/ Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia Lin, Cheow Yuen In this present study, the stem bark of Garcinia maingayi and Garcinia parvifolia were investigated and resulted in the isolation of nine compounds. There are no previous reports on chemical components and biological activities from Garcinia maingayi. The structures of these compounds were elucidated by using spectroscopic experiments namely NMR, MS, IR and UV. Being the first report on Garcinia maingayi, detailed chemical studies have afforded two triterpenoids, stigmasterol and sitosterol, two xanthones, 1,3,7-trihydroxy-2-(3- methylbut-2-enyl)-xanthone and 1,3,6,8-tetrahydroxyxanthone, one benzophenone, isoxanthochymol, and one benzoic acid derivative methyl 3,4-dihydroxybenzoate. The findings are significant as it contributes to the knowledge of the chemotaxonomy on Garcinia species and all these compounds are new to the species. iii Meanwhile investigations on Garcinia parvifolia have afforded one triterpenoid, α- amyrin and two xanthones, cowanin and rubraxanthone. Acetylation reaction was carried out on rubraxanthone to yield triacetate rubraxanthone. This is also the first report on cytotoxic and larvicidal activities of Garcinia maingayi, Garcinia parvifolia and rubraxanthone. Cytotoxic tests were performed using HL-60 and CEM-SS cell lines. The crude hexane and chloroform extracts of Garcinia maingayi were active against HL-60 cell line with IC50 values of less than 30 μg/ml. The crude hexane and acetone extracts of Garcinia parvifolia were found to be active against CEM-SS cell line with IC50 values of less than 30 μg/ml meanwhile, the crude chloroform extract gave a significant activity with an IC50 value of 6.5 μg/ml. The antimicrobial assay was carried out against four pathogenic bacteria, Methicillin resistant Staphylococcus aures, Pseudomonas aeruginosa, Staphylococcus typhimurium and Bacillus subtilis. Most of the crude extracts tested against these microbes gave only moderate or weak activity. The antifungal activity testing of the plant extracts were carried out against the fungi Candida albican, Aspergillus ochraceaus, Sacchoromyces cerevisiae and Candida lypolytica. No activity was observed for all the crude extracts The larvicidal test was carried out towards the larvae of Aedes aegypti. All the crude extracts of Garcinia maingayi were weakly active against the larvae with LC50 values of more than 150 μg/ml. The crude extracts of Garcinia parvifolia showed moderate activities against the larvae by giving LC50 values of less than 100 μg/ml. The pure iv rubraxanthone showed a strong activity against the larvae with a LC50 value of 15.49 μg/ml. 2005-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27/1/1000548961_t_FS_2005_1.pdf Lin, Cheow Yuen (2005) Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia. Masters thesis, Universiti Putra Malaysia. Garcinia - Case studies English |
| spellingShingle | Garcinia - Case studies Lin, Cheow Yuen Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia |
| title | Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
|
| title_full | Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
|
| title_fullStr | Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
|
| title_full_unstemmed | Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
|
| title_short | Chemical Constituents and Biological Activities From Garcinia Maingayi and Garcinia Parvifolia
|
| title_sort | chemical constituents and biological activities from garcinia maingayi and garcinia parvifolia |
| topic | Garcinia - Case studies |
| url | http://psasir.upm.edu.my/id/eprint/27/ http://psasir.upm.edu.my/id/eprint/27/1/1000548961_t_FS_2005_1.pdf |