Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1
The in-vitro growth of benign (EMT6) and malignant (4T1) mouse mammary tumour cell lines were evaluated following treatment with doses of 0.1, 0.01 and 0.001 mg/mL dexamethasone. The viability and growth of these cells following dexamethasone treatment were determined by cell proliferation and live:...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26851/ http://psasir.upm.edu.my/id/eprint/26851/1/PROCEEDING%2064.pdf |
| _version_ | 1848845690066698240 |
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| author | Wan Hassan, Wan Maryani Mohd Noor, Mohd Hezmee |
| author_facet | Wan Hassan, Wan Maryani Mohd Noor, Mohd Hezmee |
| author_sort | Wan Hassan, Wan Maryani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The in-vitro growth of benign (EMT6) and malignant (4T1) mouse mammary tumour cell lines were evaluated following treatment with doses of 0.1, 0.01 and 0.001 mg/mL dexamethasone. The viability and growth of these cells following dexamethasone treatment were determined by cell proliferation and live:dead assays. In cell proliferation assay, both cells were rapidly proliferating in serum-free media after treatment with different concentrations of dexamethasone over 24 hours. Following treatment with 0.1 mg/mL dexamethasone the proliferation of EMT6 cells were significantly increased while the proliferation of 4T1 cells was only slightly significantly increased. The effect was not dose-dependent. Using the live:dead assay, the results for both cell lines were consistent with the that of cell proliferation assay. This study provides preliminary evidence that dexamethasone may induce growth and viability of mouse mammary tumour cell lines, EMT6 and 4TI. |
| first_indexed | 2025-11-15T08:50:49Z |
| format | Conference or Workshop Item |
| id | upm-26851 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:50:49Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-268512016-08-01T03:41:31Z http://psasir.upm.edu.my/id/eprint/26851/ Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 Wan Hassan, Wan Maryani Mohd Noor, Mohd Hezmee The in-vitro growth of benign (EMT6) and malignant (4T1) mouse mammary tumour cell lines were evaluated following treatment with doses of 0.1, 0.01 and 0.001 mg/mL dexamethasone. The viability and growth of these cells following dexamethasone treatment were determined by cell proliferation and live:dead assays. In cell proliferation assay, both cells were rapidly proliferating in serum-free media after treatment with different concentrations of dexamethasone over 24 hours. Following treatment with 0.1 mg/mL dexamethasone the proliferation of EMT6 cells were significantly increased while the proliferation of 4T1 cells was only slightly significantly increased. The effect was not dose-dependent. Using the live:dead assay, the results for both cell lines were consistent with the that of cell proliferation assay. This study provides preliminary evidence that dexamethasone may induce growth and viability of mouse mammary tumour cell lines, EMT6 and 4TI. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26851/1/PROCEEDING%2064.pdf Wan Hassan, Wan Maryani and Mohd Noor, Mohd Hezmee (2012) Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. . |
| spellingShingle | Wan Hassan, Wan Maryani Mohd Noor, Mohd Hezmee Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title | Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title_full | Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title_fullStr | Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title_full_unstemmed | Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title_short | Effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, EMT6 and 4T1 |
| title_sort | effect of dexamethasone on growth and viability of mouse mammary tumour cell lines, emt6 and 4t1 |
| url | http://psasir.upm.edu.my/id/eprint/26851/ http://psasir.upm.edu.my/id/eprint/26851/1/PROCEEDING%2064.pdf |