Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia
Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. The PRRS virus can be divided into two antigenically and genetically different strains: Type I (European) and Typ...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26848/ http://psasir.upm.edu.my/id/eprint/26848/1/PROCEEDING%2061.pdf |
| _version_ | 1848845689220497408 |
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| author | Kiu, Vania Tse Ling Ooi, Peck Toung |
| author_facet | Kiu, Vania Tse Ling Ooi, Peck Toung |
| author_sort | Kiu, Vania Tse Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. The PRRS virus can be divided into two antigenically and genetically different strains: Type I (European) and Type II (North American). In this study, 120 sera were collected from 12 farms in 6 states in Malaysia for the seroprevalence study. Ten sera from apparently healthy sows/gilts, finishers and growers were collected from each farm and tested using IDEXX HerdChek ELISA for both strains. All the farms tested were seropositive with an overall seroprevalence of 89.2%. Tissue samples were collected and PRRSV isolated were genotyped using nested-PCR (with reported primer pairs targeting ORF7) that enabled the differentiation of Type I and Type II PRRSV by producing different sizes of PCR products. Out of 27 tissue samples collected from 11 farms, 12 were positive for PRRSV. All the PRRSV genomes from the 12 PRRSV-positive tissue homogenates were of Type II PRRSV, whereas no Type I PRRSV was detected. These data indicate that PRRS is endemic in the farms tested with a high possibility of subclinical infections. |
| first_indexed | 2025-11-15T08:50:48Z |
| format | Conference or Workshop Item |
| id | upm-26848 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:50:48Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-268482016-08-01T03:42:22Z http://psasir.upm.edu.my/id/eprint/26848/ Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia Kiu, Vania Tse Ling Ooi, Peck Toung Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. The PRRS virus can be divided into two antigenically and genetically different strains: Type I (European) and Type II (North American). In this study, 120 sera were collected from 12 farms in 6 states in Malaysia for the seroprevalence study. Ten sera from apparently healthy sows/gilts, finishers and growers were collected from each farm and tested using IDEXX HerdChek ELISA for both strains. All the farms tested were seropositive with an overall seroprevalence of 89.2%. Tissue samples were collected and PRRSV isolated were genotyped using nested-PCR (with reported primer pairs targeting ORF7) that enabled the differentiation of Type I and Type II PRRSV by producing different sizes of PCR products. Out of 27 tissue samples collected from 11 farms, 12 were positive for PRRSV. All the PRRSV genomes from the 12 PRRSV-positive tissue homogenates were of Type II PRRSV, whereas no Type I PRRSV was detected. These data indicate that PRRS is endemic in the farms tested with a high possibility of subclinical infections. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26848/1/PROCEEDING%2061.pdf Kiu, Vania Tse Ling and Ooi, Peck Toung (2012) Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. . |
| spellingShingle | Kiu, Vania Tse Ling Ooi, Peck Toung Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title | Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title_full | Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title_fullStr | Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title_full_unstemmed | Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title_short | Characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in Malaysia |
| title_sort | characterisation of porcine reproductive and respiratory syndrome virus strains in selected farms in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/26848/ http://psasir.upm.edu.my/id/eprint/26848/1/PROCEEDING%2061.pdf |