Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria
Pasteurella multocida type B is an etiological agent of haemorrhagic septicaemia in cattle and buffaloes. The disease is commonly fatal and considered as one of the most economically important cattle disease in Southeast Asia including Malaysia. This study described the detection of P. Multocida typ...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26819/ http://psasir.upm.edu.my/id/eprint/26819/1/PROCEEDING%2039.pdf |
| _version_ | 1848845680761634816 |
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| author | Mohd Yusof, Mohd Syamil Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse |
| author_facet | Mohd Yusof, Mohd Syamil Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse |
| author_sort | Mohd Yusof, Mohd Syamil |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pasteurella multocida type B is an etiological agent of haemorrhagic septicaemia in cattle and buffaloes. The disease is commonly fatal and considered as one of the most economically important cattle disease in Southeast Asia including Malaysia. This study described the detection of P. Multocida type B using Polymerase Chain Reaction (PCR) from the organs of the mice inoculated orally with the bacteria. In this study, 16 male and healthy mice aged 3-weeks were selected; they were divided into two equal groups of 8 mice each. Mice in group 1 were inoculated orally with 109 CFU of P. multocida type B, while the mice from group 2 were inoculated orally with PBS, pH 7. The mice were observed for 5 days (120 hours). Post-mortem was conducted on mice which died within 5 days of the experimental period and organs such as heart, lung liver, spleen, stomach, small intestine and large intestine were collected and subjected to isolation and identification of the bacteria on blood agar. The organs collected from the mice showed positive results using PCR which detected P. multocida type B. On the contrary, P. multocida type B was not isolated from the organs of the surviving mice which were culled at the end of the experiment. |
| first_indexed | 2025-11-15T08:50:40Z |
| format | Conference or Workshop Item |
| id | upm-26819 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:50:40Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-268192016-08-01T04:17:01Z http://psasir.upm.edu.my/id/eprint/26819/ Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria Mohd Yusof, Mohd Syamil Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse Pasteurella multocida type B is an etiological agent of haemorrhagic septicaemia in cattle and buffaloes. The disease is commonly fatal and considered as one of the most economically important cattle disease in Southeast Asia including Malaysia. This study described the detection of P. Multocida type B using Polymerase Chain Reaction (PCR) from the organs of the mice inoculated orally with the bacteria. In this study, 16 male and healthy mice aged 3-weeks were selected; they were divided into two equal groups of 8 mice each. Mice in group 1 were inoculated orally with 109 CFU of P. multocida type B, while the mice from group 2 were inoculated orally with PBS, pH 7. The mice were observed for 5 days (120 hours). Post-mortem was conducted on mice which died within 5 days of the experimental period and organs such as heart, lung liver, spleen, stomach, small intestine and large intestine were collected and subjected to isolation and identification of the bacteria on blood agar. The organs collected from the mice showed positive results using PCR which detected P. multocida type B. On the contrary, P. multocida type B was not isolated from the organs of the surviving mice which were culled at the end of the experiment. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26819/1/PROCEEDING%2039.pdf Mohd Yusof, Mohd Syamil and Omar, Abdul Rahman and Abdullah, Faez Firdaus Jesse (2012) Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (p. 99). |
| spellingShingle | Mohd Yusof, Mohd Syamil Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title | Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title_full | Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title_fullStr | Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title_full_unstemmed | Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title_short | Polymerase chain reaction detection of Pasteurellamultocida type B in mice following oral inoculation with the bacteria |
| title_sort | polymerase chain reaction detection of pasteurellamultocida type b in mice following oral inoculation with the bacteria |
| url | http://psasir.upm.edu.my/id/eprint/26819/ http://psasir.upm.edu.my/id/eprint/26819/1/PROCEEDING%2039.pdf |