Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria
Caseous lymphadenitis (CLA) is caused by aetiological agent Corynebacterium pseudotuberculosis which is an intracellular gram-positive, facultative anaerobic small curved rod bacteria. The infection of CLA may occur through the ingestion of contaminated food with the bacteria. Hence, this study was...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26791/ http://psasir.upm.edu.my/id/eprint/26791/1/PROCEEDING%2017.pdf |
| _version_ | 1848845673199304704 |
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| author | Muhdi, Ahmad Fauzan Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse |
| author_facet | Muhdi, Ahmad Fauzan Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse |
| author_sort | Muhdi, Ahmad Fauzan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Caseous lymphadenitis (CLA) is caused by aetiological agent Corynebacterium pseudotuberculosis which is an intracellular gram-positive, facultative anaerobic small curved rod bacteria. The infection of CLA may occur through the ingestion of contaminated food with the bacteria. Hence, this study was conducted to prove that the oral route will give same signs with other routes of transmission. This study was conducted to detect C. pseudotuberculosis using PCR from various organs in mice following oral route inoculation to demonstrate a natural route of the transmission of the bacteria in goat and sheep and the development of cheaper animal models to study C. pseudotuberculosis. A total of 8 mice were inoculated orally with the bacteria and 7 organs were collected for detection using PCR. PCR on DNA extracted using a pair of C. pseudotuberculosis specific primers was positive in some organs which were 3 samples of lymph node, 5 samples of brain and 1 sample of liver. Although the results in this study showed minimum detection of C. pseudotuberculosis in many organs, the PCR used in this study may successfully be applied for the detection and diagnosis of the bacteria in mice and oral inoculation or digestion transmission revealed presence of the bacteria and may produce effect in a chronic period. |
| first_indexed | 2025-11-15T08:50:33Z |
| format | Conference or Workshop Item |
| id | upm-26791 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:50:33Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-267912016-08-01T04:06:23Z http://psasir.upm.edu.my/id/eprint/26791/ Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria Muhdi, Ahmad Fauzan Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse Caseous lymphadenitis (CLA) is caused by aetiological agent Corynebacterium pseudotuberculosis which is an intracellular gram-positive, facultative anaerobic small curved rod bacteria. The infection of CLA may occur through the ingestion of contaminated food with the bacteria. Hence, this study was conducted to prove that the oral route will give same signs with other routes of transmission. This study was conducted to detect C. pseudotuberculosis using PCR from various organs in mice following oral route inoculation to demonstrate a natural route of the transmission of the bacteria in goat and sheep and the development of cheaper animal models to study C. pseudotuberculosis. A total of 8 mice were inoculated orally with the bacteria and 7 organs were collected for detection using PCR. PCR on DNA extracted using a pair of C. pseudotuberculosis specific primers was positive in some organs which were 3 samples of lymph node, 5 samples of brain and 1 sample of liver. Although the results in this study showed minimum detection of C. pseudotuberculosis in many organs, the PCR used in this study may successfully be applied for the detection and diagnosis of the bacteria in mice and oral inoculation or digestion transmission revealed presence of the bacteria and may produce effect in a chronic period. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26791/1/PROCEEDING%2017.pdf Muhdi, Ahmad Fauzan and Omar, Abdul Rahman and Abdullah, Faez Firdaus Jesse (2012) Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. . |
| spellingShingle | Muhdi, Ahmad Fauzan Omar, Abdul Rahman Abdullah, Faez Firdaus Jesse Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title | Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title_full | Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title_fullStr | Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title_full_unstemmed | Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title_short | Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| title_sort | polymerase chain reaction detection of corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria |
| url | http://psasir.upm.edu.my/id/eprint/26791/ http://psasir.upm.edu.my/id/eprint/26791/1/PROCEEDING%2017.pdf |