Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity.
The antimicrobial activity of N. sativa essential oil obtained by supercritical fluid extraction by carbon dioxide was investigated against Gram Positive and Gram negative strains, isolated from clinical specimens. Best conditions for Black cumin oil extraction are obtained at 400 bar, 40°C and a s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Science Publications
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26775/ http://psasir.upm.edu.my/id/eprint/26775/1/Extraction%20of%20essential%20oil%20from%20Nigella%20sativa%20using%20supercritical%20carbon%20dioxide.pdf |
| _version_ | 1848845668460789760 |
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| author | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Zamri, Hana Farizah Abdullah, Rasedee |
| author_facet | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Zamri, Hana Farizah Abdullah, Rasedee |
| author_sort | Abdullah Al-Haj, Nagi Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The antimicrobial activity of N. sativa essential oil obtained by supercritical fluid extraction by carbon dioxide was investigated against Gram Positive and Gram negative strains, isolated from clinical specimens. Best conditions for Black cumin oil extraction are obtained at 400 bar, 40°C and a solvent flow rate of 25 g min-1. The seed extracts were prepared by supercritical fluid extraction method. Filter paper discs impregnated with varying concentrations of N. sativa extract were tested by
the disk diffusion method. Methicillin Resistant Staphylococcus Aureus (MRSA) ATCC strain
(700968), E. coli ATCC strain (25922), E. coli 0157 ATCC strain (12799), Extended-Spectrum Beta-Lactamase (ESBL) Klebsiella pneumoniae ATCC strain (700603), Carbapenam Resistant acenitobacter Baumanii (CRAB) clinical strain and Vibrio cholerae 01 Ogawa and 0139 Bengal clinical strains were investigated. The inhibition zones of the Mueller Hinton agar in different extract concentrate ion showed that at 25 mg 20 μL-1, 50 mg 20 μL-1 and 100 mg 20 μL-1, the inhibition zones increased accordingly in S. aureu. However, N. sativa was found to be inactive against ESBL producers (E. coli and K. pneumoniae). |
| first_indexed | 2025-11-15T08:50:28Z |
| format | Article |
| id | upm-26775 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:50:28Z |
| publishDate | 2008 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-267752015-12-03T04:17:33Z http://psasir.upm.edu.my/id/eprint/26775/ Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Zamri, Hana Farizah Abdullah, Rasedee The antimicrobial activity of N. sativa essential oil obtained by supercritical fluid extraction by carbon dioxide was investigated against Gram Positive and Gram negative strains, isolated from clinical specimens. Best conditions for Black cumin oil extraction are obtained at 400 bar, 40°C and a solvent flow rate of 25 g min-1. The seed extracts were prepared by supercritical fluid extraction method. Filter paper discs impregnated with varying concentrations of N. sativa extract were tested by the disk diffusion method. Methicillin Resistant Staphylococcus Aureus (MRSA) ATCC strain (700968), E. coli ATCC strain (25922), E. coli 0157 ATCC strain (12799), Extended-Spectrum Beta-Lactamase (ESBL) Klebsiella pneumoniae ATCC strain (700603), Carbapenam Resistant acenitobacter Baumanii (CRAB) clinical strain and Vibrio cholerae 01 Ogawa and 0139 Bengal clinical strains were investigated. The inhibition zones of the Mueller Hinton agar in different extract concentrate ion showed that at 25 mg 20 μL-1, 50 mg 20 μL-1 and 100 mg 20 μL-1, the inhibition zones increased accordingly in S. aureu. However, N. sativa was found to be inactive against ESBL producers (E. coli and K. pneumoniae). Science Publications 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26775/1/Extraction%20of%20essential%20oil%20from%20Nigella%20sativa%20using%20supercritical%20carbon%20dioxide.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Zamri, Hana Farizah and Abdullah, Rasedee (2008) Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. American Journal of Pharmacology and Toxicology, 3 (4). pp. 225-228. ISSN 1557-4962; ESSN: 1557-4970 10.3844/ajptsp.2008.225.228 English |
| spellingShingle | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Zamri, Hana Farizah Abdullah, Rasedee Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title | Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title_full | Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title_fullStr | Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title_full_unstemmed | Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title_short | Extraction of essential oil from Nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| title_sort | extraction of essential oil from nigella sativa using supercritical carbon dioxide : study of antibacterial activity. |
| url | http://psasir.upm.edu.my/id/eprint/26775/ http://psasir.upm.edu.my/id/eprint/26775/ http://psasir.upm.edu.my/id/eprint/26775/1/Extraction%20of%20essential%20oil%20from%20Nigella%20sativa%20using%20supercritical%20carbon%20dioxide.pdf |