Immuniregulatory response following fluranthene instillation in embryonated chicken eggs
The immunotoxic effect the air pollutant, floranthene in chicken has never been documented. This study was undertaken to determine the possible immunotoxicity of fluoranthene in embryonated chicken eggs. Fifty 9-day-ole embryonated chicken eggs were used in this study where 40 were each inoculated...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26582/ http://psasir.upm.edu.my/id/eprint/26582/1/Immuniregulatory%20response%20following%20fluranthene%20instillation%20in%20embryonated%20chicken%20eggs.pdf |
| _version_ | 1848845616989339648 |
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| author | Abdul Wahid, Firhanis Mohamed Mustapha, Noordin Mazlan, Mazlina Md Isa, Nur Mahiza |
| author_facet | Abdul Wahid, Firhanis Mohamed Mustapha, Noordin Mazlan, Mazlina Md Isa, Nur Mahiza |
| author_sort | Abdul Wahid, Firhanis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The immunotoxic effect the air pollutant, floranthene in chicken has never been documented. This study was undertaken to determine the possible immunotoxicity of fluoranthene in embryonated chicken eggs. Fifty 9-day-ole embryonated chicken eggs were used in this study where 40 were each inoculated with fluoranthene at the dose of 15 mg/kg via the allantoic route. The remaining 10 eggs were inoculated with phosphate buffered saline (PBS) and acted as controls. All eggs were incubated at 37°C and candled every day for evidence of embryonic survival. Dead embryos (before 21 days old) were necropsied while the allantoic fluid and yolk collected for the determination of ND-HI titers. Chicks that hatched were sacrificed and blood was collected for ND-HI titer determination and lymphoid organs were procured for histopathology. Postmoetem findins fo fluoranthene inoculated embryos were stunted growth, generalized or localized harmorrahages especially at legs and head. Histopathologically, fluoranthene induced lymphoid hyperplasia in the thymus, spleen and bursa. Such change has led to an increase in antibody production compated to the controls. This study has provided evidence that fluoranthene may cross the egg barrier (in ovo) in avian species. |
| first_indexed | 2025-11-15T08:49:39Z |
| format | Conference or Workshop Item |
| id | upm-26582 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:49:39Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-265822016-11-21T07:48:40Z http://psasir.upm.edu.my/id/eprint/26582/ Immuniregulatory response following fluranthene instillation in embryonated chicken eggs Abdul Wahid, Firhanis Mohamed Mustapha, Noordin Mazlan, Mazlina Md Isa, Nur Mahiza The immunotoxic effect the air pollutant, floranthene in chicken has never been documented. This study was undertaken to determine the possible immunotoxicity of fluoranthene in embryonated chicken eggs. Fifty 9-day-ole embryonated chicken eggs were used in this study where 40 were each inoculated with fluoranthene at the dose of 15 mg/kg via the allantoic route. The remaining 10 eggs were inoculated with phosphate buffered saline (PBS) and acted as controls. All eggs were incubated at 37°C and candled every day for evidence of embryonic survival. Dead embryos (before 21 days old) were necropsied while the allantoic fluid and yolk collected for the determination of ND-HI titers. Chicks that hatched were sacrificed and blood was collected for ND-HI titer determination and lymphoid organs were procured for histopathology. Postmoetem findins fo fluoranthene inoculated embryos were stunted growth, generalized or localized harmorrahages especially at legs and head. Histopathologically, fluoranthene induced lymphoid hyperplasia in the thymus, spleen and bursa. Such change has led to an increase in antibody production compated to the controls. This study has provided evidence that fluoranthene may cross the egg barrier (in ovo) in avian species. 2011-01-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26582/1/Immuniregulatory%20response%20following%20fluranthene%20instillation%20in%20embryonated%20chicken%20eggs.pdf Abdul Wahid, Firhanis and Mohamed Mustapha, Noordin and Mazlan, Mazlina and Md Isa, Nur Mahiza (2011) Immuniregulatory response following fluranthene instillation in embryonated chicken eggs. In: 6th Seminar on Veterinary Sciences, 11-14 Jan. 2011, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 12-16). |
| spellingShingle | Abdul Wahid, Firhanis Mohamed Mustapha, Noordin Mazlan, Mazlina Md Isa, Nur Mahiza Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title | Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title_full | Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title_fullStr | Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title_full_unstemmed | Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title_short | Immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| title_sort | immuniregulatory response following fluranthene instillation in embryonated chicken eggs |
| url | http://psasir.upm.edu.my/id/eprint/26582/ http://psasir.upm.edu.my/id/eprint/26582/1/Immuniregulatory%20response%20following%20fluranthene%20instillation%20in%20embryonated%20chicken%20eggs.pdf |