Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
This study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocytecomplexes (COCs) were retrieved using aspiration method from 2-6...
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| Format: | Article |
| Language: | English English |
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University of Agriculture, Faisalabad
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25940/ http://psasir.upm.edu.my/id/eprint/25940/1/Structural%20changes%20in%20cattle%20immature%20oocytes%20subjected%20to%20slow%20freezing%20and%20vitrification.pdf |
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| author | Haron, Abdul Wahid Thein, Myint El-Hafez, Enas A. Abas, M. O. Mohd Azam, K. Othman, Fauziah Yusoff, Rosnina Hajarian, Hadi |
| author_facet | Haron, Abdul Wahid Thein, Myint El-Hafez, Enas A. Abas, M. O. Mohd Azam, K. Othman, Fauziah Yusoff, Rosnina Hajarian, Hadi |
| author_sort | Haron, Abdul Wahid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocytecomplexes (COCs) were retrieved using aspiration method from 2-6 mm follicles. In Experiment 1, selected oocytes were randomly divided into 4 treatment groups namely freezing solution-exposed, frozen-thawed, vitrification solution-exposed and vitrified-thawed and then oocytes abnormalities were examined under a stereomicroscope. In Experiment 2, oocytes were randomly allocated to the same grouping as experiment 1 plus control group. Following freezing or vitrification, all oocytes were fixed in glutaraldehyde and processed for transmission electron microscopy. In experiment 1, there was a higher incidence of abnormalities in the frozen-thawed and vitrified-warmed oocytes compared to those in freezing solution and vitrification solution-exposed groups (P <0.05). In experiment 2, there were marked alterations in the perivitelline space, microvilli and vesicles of frozenthawed and vitrified-warmed oocytescharacterized by loss of elasticity and integrity of cytoplasmic processes and microvilli following cooling and warming. In conclusion, ethylene glycol-based freezingand vitrification solutions are suitable choices for cryopreservation of immature oocytes and most organelles are able to retain their normal morphology followingcryopreservation and thawing processes |
| first_indexed | 2025-11-15T08:46:53Z |
| format | Article |
| id | upm-25940 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:46:53Z |
| publishDate | 2012 |
| publisher | University of Agriculture, Faisalabad |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-259402015-09-14T00:28:32Z http://psasir.upm.edu.my/id/eprint/25940/ Structural changes in cattle immature oocytes subjected to slow freezing and vitrification. Haron, Abdul Wahid Thein, Myint El-Hafez, Enas A. Abas, M. O. Mohd Azam, K. Othman, Fauziah Yusoff, Rosnina Hajarian, Hadi This study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocytecomplexes (COCs) were retrieved using aspiration method from 2-6 mm follicles. In Experiment 1, selected oocytes were randomly divided into 4 treatment groups namely freezing solution-exposed, frozen-thawed, vitrification solution-exposed and vitrified-thawed and then oocytes abnormalities were examined under a stereomicroscope. In Experiment 2, oocytes were randomly allocated to the same grouping as experiment 1 plus control group. Following freezing or vitrification, all oocytes were fixed in glutaraldehyde and processed for transmission electron microscopy. In experiment 1, there was a higher incidence of abnormalities in the frozen-thawed and vitrified-warmed oocytes compared to those in freezing solution and vitrification solution-exposed groups (P <0.05). In experiment 2, there were marked alterations in the perivitelline space, microvilli and vesicles of frozenthawed and vitrified-warmed oocytescharacterized by loss of elasticity and integrity of cytoplasmic processes and microvilli following cooling and warming. In conclusion, ethylene glycol-based freezingand vitrification solutions are suitable choices for cryopreservation of immature oocytes and most organelles are able to retain their normal morphology followingcryopreservation and thawing processes University of Agriculture, Faisalabad 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25940/1/Structural%20changes%20in%20cattle%20immature%20oocytes%20subjected%20to%20slow%20freezing%20and%20vitrification.pdf Haron, Abdul Wahid and Thein, Myint and El-Hafez, Enas A. and Abas, M. O. and Mohd Azam, K. and Othman, Fauziah and Yusoff, Rosnina and Hajarian, Hadi (2012) Structural changes in cattle immature oocytes subjected to slow freezing and vitrification. Pakistan Veterinary Journal, 32 (2). pp. 188-192. ISSN 0253-8318; ESSN: 2074-7764 English |
| spellingShingle | Haron, Abdul Wahid Thein, Myint El-Hafez, Enas A. Abas, M. O. Mohd Azam, K. Othman, Fauziah Yusoff, Rosnina Hajarian, Hadi Structural changes in cattle immature oocytes subjected to slow freezing and vitrification. |
| title | Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
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| title_full | Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
|
| title_fullStr | Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
|
| title_full_unstemmed | Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
|
| title_short | Structural changes in cattle immature oocytes subjected to slow freezing and vitrification.
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| title_sort | structural changes in cattle immature oocytes subjected to slow freezing and vitrification. |
| url | http://psasir.upm.edu.my/id/eprint/25940/ http://psasir.upm.edu.my/id/eprint/25940/1/Structural%20changes%20in%20cattle%20immature%20oocytes%20subjected%20to%20slow%20freezing%20and%20vitrification.pdf |