Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.

A study was carried out to isolate Brucella melitensis using established bacteriological techniques and the Polymerase Chain Reaction in previously known positive goat farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang states of Malaysia. A total of 7 isolates were recovered from vaginal sw...

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Main Authors: Bamaiyi , Pwaveno Huladeino, Hassan, Latiffah, Bejo, Siti Khairani, Mohamed, Zainal Abidin, Mahmod, Ramlan, Krishnan, N., Adzhar, A., Abdullah, N., N.H.M, Hamidah, M.M, Norsuhanna, Hashim, S.N.
Format: Conference or Workshop Item
Language:English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25707/
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author Bamaiyi , Pwaveno Huladeino
Hassan, Latiffah
Bejo, Siti Khairani
Mohamed, Zainal Abidin
Mahmod, Ramlan
Krishnan, N.
Adzhar, A.
Abdullah, N.
N.H.M, Hamidah
M.M, Norsuhanna
Hashim, S.N.
author_facet Bamaiyi , Pwaveno Huladeino
Hassan, Latiffah
Bejo, Siti Khairani
Mohamed, Zainal Abidin
Mahmod, Ramlan
Krishnan, N.
Adzhar, A.
Abdullah, N.
N.H.M, Hamidah
M.M, Norsuhanna
Hashim, S.N.
author_sort Bamaiyi , Pwaveno Huladeino
building UPM Institutional Repository
collection Online Access
description A study was carried out to isolate Brucella melitensis using established bacteriological techniques and the Polymerase Chain Reaction in previously known positive goat farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang states of Malaysia. A total of 7 isolates were recovered from vaginal swabs, spleen and uterine fluid using standard biochemical tests with vaginal swabs yielding the highest isolates of 4 (57.14%) followed by the spleen 2 (28.57%) and uterine fluid 1(14.29%). No isolates were recovered from the lymph nodes. PCR confirmed all 7 isolates as B. melitensis
first_indexed 2025-11-15T08:45:51Z
format Conference or Workshop Item
id upm-25707
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T08:45:51Z
publishDate 2011
recordtype eprints
repository_type Digital Repository
spelling upm-257072013-10-11T08:04:10Z http://psasir.upm.edu.my/id/eprint/25707/ Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia. Bamaiyi , Pwaveno Huladeino Hassan, Latiffah Bejo, Siti Khairani Mohamed, Zainal Abidin Mahmod, Ramlan Krishnan, N. Adzhar, A. Abdullah, N. N.H.M, Hamidah M.M, Norsuhanna Hashim, S.N. A study was carried out to isolate Brucella melitensis using established bacteriological techniques and the Polymerase Chain Reaction in previously known positive goat farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang states of Malaysia. A total of 7 isolates were recovered from vaginal swabs, spleen and uterine fluid using standard biochemical tests with vaginal swabs yielding the highest isolates of 4 (57.14%) followed by the spleen 2 (28.57%) and uterine fluid 1(14.29%). No isolates were recovered from the lymph nodes. PCR confirmed all 7 isolates as B. melitensis 2011 Conference or Workshop Item PeerReviewed Bamaiyi , Pwaveno Huladeino and Hassan, Latiffah and Bejo, Siti Khairani and Mohamed, Zainal Abidin and Mahmod, Ramlan and Krishnan, N. and Adzhar, A. and Abdullah, N. and N.H.M, Hamidah and M.M, Norsuhanna and Hashim, S.N. (2011) Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia. In: 2nd International Symposium on Zoonoses and Emerging Infectious Diseases , 2011, Kuala Lumpur, Malaysia. (p. 30). English
spellingShingle Bamaiyi , Pwaveno Huladeino
Hassan, Latiffah
Bejo, Siti Khairani
Mohamed, Zainal Abidin
Mahmod, Ramlan
Krishnan, N.
Adzhar, A.
Abdullah, N.
N.H.M, Hamidah
M.M, Norsuhanna
Hashim, S.N.
Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title_full Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title_fullStr Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title_full_unstemmed Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title_short Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
title_sort isolation and polymerase chain reaction confirmation of brucella melitensis from goats in malaysia.
url http://psasir.upm.edu.my/id/eprint/25707/