Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)

The extracts of ten culinary spices were screened to identify their antimicrobial activities against Streptococcus agalactiae by using disk diffusion assay. Only Cinnamomum verum, Allium sativum Linn, Eugenia caryophyllus and Thymus vulgaris displayed antimicrobial activity. The bark C. verum extrac...

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Main Authors: Alsaid, Milud, Mohd Daud, Hassan, Bejo, Siti Khairani, Abuseliana, Ali
Format: Article
Language:English
Published: IDOSI Publications 2010
Online Access:http://psasir.upm.edu.my/id/eprint/25700/
http://psasir.upm.edu.my/id/eprint/25700/1/Antimicrobial%20activities%20of%20some%20culinary%20spice%20extracts%20against%20Streptococcus%20agalactiae%20and%20its%20prophylactic%20uses%20to%20prevent%20streptococcal%20infection%20in%20red%20hybrid%20tilapia.pdf
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author Alsaid, Milud
Mohd Daud, Hassan
Bejo, Siti Khairani
Abuseliana, Ali
author_facet Alsaid, Milud
Mohd Daud, Hassan
Bejo, Siti Khairani
Abuseliana, Ali
author_sort Alsaid, Milud
building UPM Institutional Repository
collection Online Access
description The extracts of ten culinary spices were screened to identify their antimicrobial activities against Streptococcus agalactiae by using disk diffusion assay. Only Cinnamomum verum, Allium sativum Linn, Eugenia caryophyllus and Thymus vulgaris displayed antimicrobial activity. The bark C. verum extract displayed the highest antimicrobial activity with a 18 mm inhibition zone. The minimum inhibitory concentration (MIC) values for spice extracts were determined by utilizing the agar diffusion method. The lowest MIC value with high efficacy against S. agalactiae was 0.15 mg\mL, which was obtained from C. verum extract. The median lethal dose (LD50) of S. agalactiae to tilapia fingerlings was measured to be 1.56×105 CFU/mL. The in vivo antimicrobial effect of C. verum was tested by feeding tilapia fingerlings fish feed supplemented with different ratios of C. verum extract and bark powder for 17 days after experimentally injecting the fish with S. agalactiae intraperitoneally (IP). The mortality was significantly lower (p<0.05) in the fish fed on feed supplemented with bark C. verum extract with a ratio of 3:26 (w/w) compared to other groups. These results indicated that the C. verum bark extract supplement is promising as a prophylactic against tilapia streptococcosis and for fish health improvement.
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spelling upm-257002017-10-31T05:21:49Z http://psasir.upm.edu.my/id/eprint/25700/ Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.) Alsaid, Milud Mohd Daud, Hassan Bejo, Siti Khairani Abuseliana, Ali The extracts of ten culinary spices were screened to identify their antimicrobial activities against Streptococcus agalactiae by using disk diffusion assay. Only Cinnamomum verum, Allium sativum Linn, Eugenia caryophyllus and Thymus vulgaris displayed antimicrobial activity. The bark C. verum extract displayed the highest antimicrobial activity with a 18 mm inhibition zone. The minimum inhibitory concentration (MIC) values for spice extracts were determined by utilizing the agar diffusion method. The lowest MIC value with high efficacy against S. agalactiae was 0.15 mg\mL, which was obtained from C. verum extract. The median lethal dose (LD50) of S. agalactiae to tilapia fingerlings was measured to be 1.56×105 CFU/mL. The in vivo antimicrobial effect of C. verum was tested by feeding tilapia fingerlings fish feed supplemented with different ratios of C. verum extract and bark powder for 17 days after experimentally injecting the fish with S. agalactiae intraperitoneally (IP). The mortality was significantly lower (p<0.05) in the fish fed on feed supplemented with bark C. verum extract with a ratio of 3:26 (w/w) compared to other groups. These results indicated that the C. verum bark extract supplement is promising as a prophylactic against tilapia streptococcosis and for fish health improvement. IDOSI Publications 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25700/1/Antimicrobial%20activities%20of%20some%20culinary%20spice%20extracts%20against%20Streptococcus%20agalactiae%20and%20its%20prophylactic%20uses%20to%20prevent%20streptococcal%20infection%20in%20red%20hybrid%20tilapia.pdf Alsaid, Milud and Mohd Daud, Hassan and Bejo, Siti Khairani and Abuseliana, Ali (2010) Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.). World Journal of Fish and Marine Sciences, 2 (6). pp. 532-538. ISSN 2078-4589; ESSN: 2078-4600 https://www.idosi.org/wjfms/wjfms2(6)10.htm
spellingShingle Alsaid, Milud
Mohd Daud, Hassan
Bejo, Siti Khairani
Abuseliana, Ali
Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title_full Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title_fullStr Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title_full_unstemmed Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title_short Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.)
title_sort antimicrobial activities of some culinary spice extracts against streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (oreochromis sp.)
url http://psasir.upm.edu.my/id/eprint/25700/
http://psasir.upm.edu.my/id/eprint/25700/
http://psasir.upm.edu.my/id/eprint/25700/1/Antimicrobial%20activities%20of%20some%20culinary%20spice%20extracts%20against%20Streptococcus%20agalactiae%20and%20its%20prophylactic%20uses%20to%20prevent%20streptococcal%20infection%20in%20red%20hybrid%20tilapia.pdf