Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia

The influence of soil texture (silt, sand and laterite) and flotation solutions (saturated NaCl, sucrose, NaNO3 and ZnSO4) upon the recovery of Toxocara ova from seeded soil samples with the centrifugal flotation technique was investigated. Soil samples of different texture were artificially seeded...

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Main Authors: Loh, A. G., Israf Ali, Daud Ahmad
Format: Article
Language:English
Published: Cambridge University Press 1998
Online Access:http://psasir.upm.edu.my/id/eprint/25577/
http://psasir.upm.edu.my/id/eprint/25577/1/Tests%20on%20the%20centrifugal%20flotation%20technique%20and%20its%20use%20in%20estimating%20the%20prevalence%20of%20Toxocara%20in%20soil%20samples%20from%20urban%20and%20suburban%20areas%20of%20Malaysia.pdf
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author Loh, A. G.
Israf Ali, Daud Ahmad
author_facet Loh, A. G.
Israf Ali, Daud Ahmad
author_sort Loh, A. G.
building UPM Institutional Repository
collection Online Access
description The influence of soil texture (silt, sand and laterite) and flotation solutions (saturated NaCl, sucrose, NaNO3 and ZnSO4) upon the recovery of Toxocara ova from seeded soil samples with the centrifugal flotation technique was investigated. Soil samples of different texture were artificially seeded with Toxocara spp. ova and subjected to a centrifugal flotation technique which used various flotation solutions. The results showed significant (P<0.001) interactions between the soil types and the flotation solutions. The highest percentage of ova recovery was obtained with silty soil (34.9−100.8%) with saturated NaC1 as the flotation solution (45.3−100.8%). A combination of washing of soil samples with 0.1% Tween 80, and flotation using saturated NaCl and a 30 min coverslip recovery period was used to study the prevalence of contamination of soil samples. Forty-six soil samples were collected from up to 24 public parks/playgrounds in urban areas of Petaling Jaya and suburban areas of Serdang. The prevalence of Toxocara species in the urban and suburban areas was 54.5% and 45.8% respectively.
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spelling upm-255772019-10-24T08:12:57Z http://psasir.upm.edu.my/id/eprint/25577/ Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia Loh, A. G. Israf Ali, Daud Ahmad The influence of soil texture (silt, sand and laterite) and flotation solutions (saturated NaCl, sucrose, NaNO3 and ZnSO4) upon the recovery of Toxocara ova from seeded soil samples with the centrifugal flotation technique was investigated. Soil samples of different texture were artificially seeded with Toxocara spp. ova and subjected to a centrifugal flotation technique which used various flotation solutions. The results showed significant (P<0.001) interactions between the soil types and the flotation solutions. The highest percentage of ova recovery was obtained with silty soil (34.9−100.8%) with saturated NaC1 as the flotation solution (45.3−100.8%). A combination of washing of soil samples with 0.1% Tween 80, and flotation using saturated NaCl and a 30 min coverslip recovery period was used to study the prevalence of contamination of soil samples. Forty-six soil samples were collected from up to 24 public parks/playgrounds in urban areas of Petaling Jaya and suburban areas of Serdang. The prevalence of Toxocara species in the urban and suburban areas was 54.5% and 45.8% respectively. Cambridge University Press 1998-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25577/1/Tests%20on%20the%20centrifugal%20flotation%20technique%20and%20its%20use%20in%20estimating%20the%20prevalence%20of%20Toxocara%20in%20soil%20samples%20from%20urban%20and%20suburban%20areas%20of%20Malaysia.pdf Loh, A. G. and Israf Ali, Daud Ahmad (1998) Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia. Journal of Helminthology, 72 (1). pp. 39-42. ISSN 0022-149X; ESSN: 1475-2697 https://www.cambridge.org/core/journals/journal-of-helminthology/article/tests-on-the-centrifugal-flotation-technique-and-its-use-in-estimating-the-prevalence-of-toxocara-in-soil-samples-from-urban-and-suburban-areas-of-malaysia/0D4589E4BFDC5CA0F57F040B66 10.1017/S0022149X0000095X
spellingShingle Loh, A. G.
Israf Ali, Daud Ahmad
Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title_full Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title_fullStr Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title_full_unstemmed Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title_short Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia
title_sort tests on the centrifugal flotation technique and its use in estimating the prevalence of toxocara in soil samples from urban and suburban areas of malaysia
url http://psasir.upm.edu.my/id/eprint/25577/
http://psasir.upm.edu.my/id/eprint/25577/
http://psasir.upm.edu.my/id/eprint/25577/
http://psasir.upm.edu.my/id/eprint/25577/1/Tests%20on%20the%20centrifugal%20flotation%20technique%20and%20its%20use%20in%20estimating%20the%20prevalence%20of%20Toxocara%20in%20soil%20samples%20from%20urban%20and%20suburban%20areas%20of%20Malaysia.pdf