Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
Context: Conjugated linoleic acids (CLAs) are a mixture of positional and geometric isomers of linoleic acid (LA) and believed to have many positive biological activities. Objective: The present study was undertaken to assess the antioxidant activity of cis-9, trans-11 and trans-10, cis-12 as single...
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| Format: | Article |
| Language: | English English |
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Informa Healthcare
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25553/ http://psasir.upm.edu.my/id/eprint/25553/1/Free%20radical%20scavenging%20activity%20of%20conjugated%20linoleic%20acid%20as%20single%20or%20mixed%20isomers.pdf |
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| author | Mohammed Ali, Yassir Abdul Kadir, Arifah Ahmad, Zuraini Yaakub, Halimatun Zakaria, Zainul Amiruddin Abdullah, Muhammed Nazrul Hakim |
| author_facet | Mohammed Ali, Yassir Abdul Kadir, Arifah Ahmad, Zuraini Yaakub, Halimatun Zakaria, Zainul Amiruddin Abdullah, Muhammed Nazrul Hakim |
| author_sort | Mohammed Ali, Yassir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Context: Conjugated linoleic acids (CLAs) are a mixture of positional and geometric isomers of linoleic acid (LA) and believed to have many positive biological activities. Objective: The present study was undertaken to assess the antioxidant activity of cis-9, trans-11 and trans-10, cis-12 as single or mixed CLA isomers at two ratios, 1:6 and 1:13 (trans-10, cis-12/cis-9, trans-11). Materials and methods: A microplate reader was used to determine the free radical scavenging properties of CLAs against DPPH radical in ethanol. Results: The kinetic reactions of CLA-DPPH• showed that all tested CLAs have exerted radical scavenging activities in a dose-dependent manner and observed to immediately react and quench DPPH radicals at all tested levels and no lag phase was noticed in CLA-DPPH• reactions. The median inhibitory concentration (IC50) value for cis-9, trans-11 CLA was observed to be more effective than other tested CLA. Total antioxidant capacity (TAC) of all tested CLAs were less effective radical scavengers as compared to vitamin E and butylated hydroxytoluene, although all tested CLAs were quenched a high amount (P<0.05) of DPPH free radicals. Discussion and conclusion: All tested CLAs have the ability to directly react and quench DPPH free radicals in ethanol. Furthermore, trans-10, cis-12 CLA has greater maximal efficacy than other tested CLAs as free radical scavenger, while cis-9, trans-11 CLA is the most potent isomer to directly react and quench free radicals at low concentrations in the system, suggesting that the free radical scavenging activity of CLA isomers may contribute to their diverse biological activities. |
| first_indexed | 2025-11-15T08:45:15Z |
| format | Article |
| id | upm-25553 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:45:15Z |
| publishDate | 2012 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-255532015-10-09T08:02:56Z http://psasir.upm.edu.my/id/eprint/25553/ Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers. Mohammed Ali, Yassir Abdul Kadir, Arifah Ahmad, Zuraini Yaakub, Halimatun Zakaria, Zainul Amiruddin Abdullah, Muhammed Nazrul Hakim Context: Conjugated linoleic acids (CLAs) are a mixture of positional and geometric isomers of linoleic acid (LA) and believed to have many positive biological activities. Objective: The present study was undertaken to assess the antioxidant activity of cis-9, trans-11 and trans-10, cis-12 as single or mixed CLA isomers at two ratios, 1:6 and 1:13 (trans-10, cis-12/cis-9, trans-11). Materials and methods: A microplate reader was used to determine the free radical scavenging properties of CLAs against DPPH radical in ethanol. Results: The kinetic reactions of CLA-DPPH• showed that all tested CLAs have exerted radical scavenging activities in a dose-dependent manner and observed to immediately react and quench DPPH radicals at all tested levels and no lag phase was noticed in CLA-DPPH• reactions. The median inhibitory concentration (IC50) value for cis-9, trans-11 CLA was observed to be more effective than other tested CLA. Total antioxidant capacity (TAC) of all tested CLAs were less effective radical scavengers as compared to vitamin E and butylated hydroxytoluene, although all tested CLAs were quenched a high amount (P<0.05) of DPPH free radicals. Discussion and conclusion: All tested CLAs have the ability to directly react and quench DPPH free radicals in ethanol. Furthermore, trans-10, cis-12 CLA has greater maximal efficacy than other tested CLAs as free radical scavenger, while cis-9, trans-11 CLA is the most potent isomer to directly react and quench free radicals at low concentrations in the system, suggesting that the free radical scavenging activity of CLA isomers may contribute to their diverse biological activities. Informa Healthcare 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25553/1/Free%20radical%20scavenging%20activity%20of%20conjugated%20linoleic%20acid%20as%20single%20or%20mixed%20isomers.pdf Mohammed Ali, Yassir and Abdul Kadir, Arifah and Ahmad, Zuraini and Yaakub, Halimatun and Zakaria, Zainul Amiruddin and Abdullah, Muhammed Nazrul Hakim (2012) Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers. Pharmaceutical Biology, 50 (6). pp. 712-719. ISSN 1388-0209 10.3109/13880209.2011.621714 English |
| spellingShingle | Mohammed Ali, Yassir Abdul Kadir, Arifah Ahmad, Zuraini Yaakub, Halimatun Zakaria, Zainul Amiruddin Abdullah, Muhammed Nazrul Hakim Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers. |
| title | Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
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| title_full | Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
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| title_fullStr | Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
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| title_full_unstemmed | Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
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| title_short | Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers.
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| title_sort | free radical scavenging activity of conjugated linoleic acid as single or mixed isomers. |
| url | http://psasir.upm.edu.my/id/eprint/25553/ http://psasir.upm.edu.my/id/eprint/25553/ http://psasir.upm.edu.my/id/eprint/25553/1/Free%20radical%20scavenging%20activity%20of%20conjugated%20linoleic%20acid%20as%20single%20or%20mixed%20isomers.pdf |