Molecular characterization of Pasteurella multocida isolates from rabbits
Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and...
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| Format: | Article |
| Language: | English English |
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Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25446/ http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf |
| _version_ | 1848845311348310016 |
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| author | Al-Haddawi, Muthafar Hussain Jasni, Sabri Radu, Son Mutalib, Abdul Rahim Bahaman, Abdul Rani Saad, Mohd Zamri Sheikh Omar, Abdul Rahman |
| author_facet | Al-Haddawi, Muthafar Hussain Jasni, Sabri Radu, Son Mutalib, Abdul Rahim Bahaman, Abdul Rani Saad, Mohd Zamri Sheikh Omar, Abdul Rahman |
| author_sort | Al-Haddawi, Muthafar Hussain |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and somatic type, and the anatomic site of isolation. Six isolates were found harboring plasmids. A similar ladder pattern ranging from 18 to 3 megadalton (Mda) were found in three isolates recovered from diseased rabbits. One band of molecular weight 6.6 Mda was shared by four of five (4/5) isolates from the diseased rabbits. No correlation was found between the presence of the common plasmids and serotype, resistance to antimicrobial agents, and anatomic sites from which the bacteria were cultured. Random amplification polymorphic DNA was applied to subtype all the isolates of P. multocida. Two single primers were tested for their abilities to generate individual fingerprints by using PCR. Primer 1 grouped the isolates into 7 profiles, and primer 2 grouped them into 15. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) results show the presence of a wide heterogeneity within P. multocida isolates. Therefore RAPD-PCR is an efficient technique to detect the DNA polymorphism and could be used to discriminate P. multocida of rabbit isolates together with serologic typing. |
| first_indexed | 2025-11-15T08:44:48Z |
| format | Article |
| id | upm-25446 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:44:48Z |
| publishDate | 1999 |
| publisher | Applied Microbiology, Molecular and Cellular Biosciences Research Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-254462024-08-07T07:29:48Z http://psasir.upm.edu.my/id/eprint/25446/ Molecular characterization of Pasteurella multocida isolates from rabbits Al-Haddawi, Muthafar Hussain Jasni, Sabri Radu, Son Mutalib, Abdul Rahim Bahaman, Abdul Rani Saad, Mohd Zamri Sheikh Omar, Abdul Rahman Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and somatic type, and the anatomic site of isolation. Six isolates were found harboring plasmids. A similar ladder pattern ranging from 18 to 3 megadalton (Mda) were found in three isolates recovered from diseased rabbits. One band of molecular weight 6.6 Mda was shared by four of five (4/5) isolates from the diseased rabbits. No correlation was found between the presence of the common plasmids and serotype, resistance to antimicrobial agents, and anatomic sites from which the bacteria were cultured. Random amplification polymorphic DNA was applied to subtype all the isolates of P. multocida. Two single primers were tested for their abilities to generate individual fingerprints by using PCR. Primer 1 grouped the isolates into 7 profiles, and primer 2 grouped them into 15. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) results show the presence of a wide heterogeneity within P. multocida isolates. Therefore RAPD-PCR is an efficient technique to detect the DNA polymorphism and could be used to discriminate P. multocida of rabbit isolates together with serologic typing. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf text en http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf Al-Haddawi, Muthafar Hussain and Jasni, Sabri and Radu, Son and Mutalib, Abdul Rahim and Bahaman, Abdul Rani and Saad, Mohd Zamri and Sheikh Omar, Abdul Rahman (1999) Molecular characterization of Pasteurella multocida isolates from rabbits. Journal of General and Applied Microbiology, 45 (6). pp. 269-275. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam/45/6/45_6_269/_article 10.2323/jgam.45.269 |
| spellingShingle | Al-Haddawi, Muthafar Hussain Jasni, Sabri Radu, Son Mutalib, Abdul Rahim Bahaman, Abdul Rani Saad, Mohd Zamri Sheikh Omar, Abdul Rahman Molecular characterization of Pasteurella multocida isolates from rabbits |
| title | Molecular characterization of Pasteurella multocida isolates from rabbits |
| title_full | Molecular characterization of Pasteurella multocida isolates from rabbits |
| title_fullStr | Molecular characterization of Pasteurella multocida isolates from rabbits |
| title_full_unstemmed | Molecular characterization of Pasteurella multocida isolates from rabbits |
| title_short | Molecular characterization of Pasteurella multocida isolates from rabbits |
| title_sort | molecular characterization of pasteurella multocida isolates from rabbits |
| url | http://psasir.upm.edu.my/id/eprint/25446/ http://psasir.upm.edu.my/id/eprint/25446/ http://psasir.upm.edu.my/id/eprint/25446/ http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf |