Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus)
The intestinal microflora of snakehead (Channa striatus) fish was studied to isolate and identify lactic acid bacteria as new probiotic. A total of five lactic acid bacteria were isolated from intestine to evaluate with probiotic properties. Leuconostoc mesenteroides sp. mesenteroides with more abil...
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| Format: | Article |
| Language: | English |
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Academic Journals
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25424/ http://psasir.upm.edu.my/id/eprint/25424/1/25424.pdf |
| _version_ | 1848845306556317696 |
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| author | Allameh, Sayyed Kamaleddin Mohd Daud, Hassan Md Yusoff, Fatimah Saad, Che Roos Ideris, Aini |
| author_facet | Allameh, Sayyed Kamaleddin Mohd Daud, Hassan Md Yusoff, Fatimah Saad, Che Roos Ideris, Aini |
| author_sort | Allameh, Sayyed Kamaleddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The intestinal microflora of snakehead (Channa striatus) fish was studied to isolate and identify lactic acid bacteria as new probiotic. A total of five lactic acid bacteria were isolated from intestine to evaluate with probiotic properties. Leuconostoc mesenteroides sp. mesenteroides with more ability to inhibit growth of Aeromonas hydrophila was selected and identified by conventional and molecular techniques. This strain was able to survive and grow from pH 3 to 8 with the highest viability and growth rate at neutral conditions (pH 7). In addition, L. mesenteroides tolerated 0, 0.15 and 0.3% bile salt concentrations. This bacterium also, showed inhibitory activity against three tested fish pathogens which included; A. hydrophila, Pseudomonas aeruginosa and Shewanella putrefaciens. Antibiotic sensitivity test indicated that this strain was resistant to Streptomycin, intermediate to Amoxicillin and Kanamycin and sensitive to Gentamycin, Tetracycline, Chloramphenicol, and Ampicillin. |
| first_indexed | 2025-11-15T08:44:43Z |
| format | Article |
| id | upm-25424 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:44:43Z |
| publishDate | 2012 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-254242016-05-31T09:13:51Z http://psasir.upm.edu.my/id/eprint/25424/ Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) Allameh, Sayyed Kamaleddin Mohd Daud, Hassan Md Yusoff, Fatimah Saad, Che Roos Ideris, Aini The intestinal microflora of snakehead (Channa striatus) fish was studied to isolate and identify lactic acid bacteria as new probiotic. A total of five lactic acid bacteria were isolated from intestine to evaluate with probiotic properties. Leuconostoc mesenteroides sp. mesenteroides with more ability to inhibit growth of Aeromonas hydrophila was selected and identified by conventional and molecular techniques. This strain was able to survive and grow from pH 3 to 8 with the highest viability and growth rate at neutral conditions (pH 7). In addition, L. mesenteroides tolerated 0, 0.15 and 0.3% bile salt concentrations. This bacterium also, showed inhibitory activity against three tested fish pathogens which included; A. hydrophila, Pseudomonas aeruginosa and Shewanella putrefaciens. Antibiotic sensitivity test indicated that this strain was resistant to Streptomycin, intermediate to Amoxicillin and Kanamycin and sensitive to Gentamycin, Tetracycline, Chloramphenicol, and Ampicillin. Academic Journals 2012-02-23 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25424/1/25424.pdf Allameh, Sayyed Kamaleddin and Mohd Daud, Hassan and Md Yusoff, Fatimah and Saad, Che Roos and Ideris, Aini (2012) Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus). African Journal of Biotechnology, 11 (16). art. no. 2E0F07C39563. pp. 3810-3816. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/2E0F07C39563 10.5897/AJB11.1871 |
| spellingShingle | Allameh, Sayyed Kamaleddin Mohd Daud, Hassan Md Yusoff, Fatimah Saad, Che Roos Ideris, Aini Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title | Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title_full | Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title_fullStr | Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title_full_unstemmed | Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title_short | Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channa striatus) |
| title_sort | isolation, identification and characterization of leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (channa striatus) |
| url | http://psasir.upm.edu.my/id/eprint/25424/ http://psasir.upm.edu.my/id/eprint/25424/ http://psasir.upm.edu.my/id/eprint/25424/ http://psasir.upm.edu.my/id/eprint/25424/1/25424.pdf |