Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats

The aim of this research was to determine the effects of Momordica charantia (MC) fruit aqueous extract on pancreatic histopathological changes in neonatal STZ-induced type-II diabetic rats. Diabetes mellitus was induced in one day Sprague-Dawley neonatal rats using a single intrapretoneal injection...

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Main Authors: Abdollahi, Maryam, Abu Bakar @ Zakaria, Md Zuki, Goh, Yong Meng, Rezaeizadeh, Alireza, Mohamed Mustapha, Noordin
Format: Article
Language:English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25418/
http://psasir.upm.edu.my/id/eprint/25418/1/Effects%20of%20momordica%20charantiaon%20pancreatic%20histopathological%20changes%20associated%20with%20streptozotocin.pdf
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author Abdollahi, Maryam
Abu Bakar @ Zakaria, Md Zuki
Goh, Yong Meng
Rezaeizadeh, Alireza
Mohamed Mustapha, Noordin
author_facet Abdollahi, Maryam
Abu Bakar @ Zakaria, Md Zuki
Goh, Yong Meng
Rezaeizadeh, Alireza
Mohamed Mustapha, Noordin
author_sort Abdollahi, Maryam
building UPM Institutional Repository
collection Online Access
description The aim of this research was to determine the effects of Momordica charantia (MC) fruit aqueous extract on pancreatic histopathological changes in neonatal STZ-induced type-II diabetic rats. Diabetes mellitus was induced in one day Sprague-Dawley neonatal rats using a single intrapretoneal injection of streptozotocin (STZ) (85 mg/kg body weight) and monitored for 12 weeks thereafter. The diabetic rats were separated into three groups, as follows: the diabetic control group (i.e. nSTZ), the diabetic group (i.e. nSTZ/M) - which was orally given 20 mg/kg of MC fruit extract, and the diabetic group (i.e. nSTZ/G) - that was treated with glibenclamide, 0.1 mg/kg for a period of four weeks. At the end of treatment, the animals were sacrificed and blood samples were collected from the saphenous vein to measure the blood glucose and serum insulin level. The pancreatic specimens were removed and processed for light microscopy, electron microscopy examination and immunohistochemical study. The results of this study showed that MC fruit aqueous extract reduced the blood glucose level as well as glibenclamide and increased the serum insulin level in the treated diabetic rats (P<0.05). The fruit extract of MC alleviated pancreatic damage and increased the number of ß-cells in the diabetic treated rats (P<0.05). Our results suggest that oral feeding of MC fruit extract may have a significant role in the renewal of pancreatic ß-cells in the nSTZ rats.
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spelling upm-254182016-11-16T09:13:42Z http://psasir.upm.edu.my/id/eprint/25418/ Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats Abdollahi, Maryam Abu Bakar @ Zakaria, Md Zuki Goh, Yong Meng Rezaeizadeh, Alireza Mohamed Mustapha, Noordin The aim of this research was to determine the effects of Momordica charantia (MC) fruit aqueous extract on pancreatic histopathological changes in neonatal STZ-induced type-II diabetic rats. Diabetes mellitus was induced in one day Sprague-Dawley neonatal rats using a single intrapretoneal injection of streptozotocin (STZ) (85 mg/kg body weight) and monitored for 12 weeks thereafter. The diabetic rats were separated into three groups, as follows: the diabetic control group (i.e. nSTZ), the diabetic group (i.e. nSTZ/M) - which was orally given 20 mg/kg of MC fruit extract, and the diabetic group (i.e. nSTZ/G) - that was treated with glibenclamide, 0.1 mg/kg for a period of four weeks. At the end of treatment, the animals were sacrificed and blood samples were collected from the saphenous vein to measure the blood glucose and serum insulin level. The pancreatic specimens were removed and processed for light microscopy, electron microscopy examination and immunohistochemical study. The results of this study showed that MC fruit aqueous extract reduced the blood glucose level as well as glibenclamide and increased the serum insulin level in the treated diabetic rats (P<0.05). The fruit extract of MC alleviated pancreatic damage and increased the number of ß-cells in the diabetic treated rats (P<0.05). Our results suggest that oral feeding of MC fruit extract may have a significant role in the renewal of pancreatic ß-cells in the nSTZ rats. 2011-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25418/1/Effects%20of%20momordica%20charantiaon%20pancreatic%20histopathological%20changes%20associated%20with%20streptozotocin.pdf Abdollahi, Maryam and Abu Bakar @ Zakaria, Md Zuki and Goh, Yong Meng and Rezaeizadeh, Alireza and Mohamed Mustapha, Noordin (2011) Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats. Histology and Hystopathology, 26 (1). pp. 13-21. ISSN 0213-3911; ESSN: 1699-5848 http://www.hh.um.es/Abstracts/Vol_26/26_1/26_1_13.htm
spellingShingle Abdollahi, Maryam
Abu Bakar @ Zakaria, Md Zuki
Goh, Yong Meng
Rezaeizadeh, Alireza
Mohamed Mustapha, Noordin
Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title_full Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title_fullStr Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title_full_unstemmed Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title_short Effects of Momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
title_sort effects of momordica charantiaon pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats
url http://psasir.upm.edu.my/id/eprint/25418/
http://psasir.upm.edu.my/id/eprint/25418/
http://psasir.upm.edu.my/id/eprint/25418/1/Effects%20of%20momordica%20charantiaon%20pancreatic%20histopathological%20changes%20associated%20with%20streptozotocin.pdf