Detection of Brucella melitensis in seropositive goats.
This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and sub...
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| Format: | Article |
| Language: | English English |
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Online Journal of Veterinary Research
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25417/ http://psasir.upm.edu.my/id/eprint/25417/1/Detection%20of%20Brucella%20melitensis%20in%20seropositive%20goats.pdf |
| _version_ | 1848845304698241024 |
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| author | H., Plumeriastuti Saad, Mohd Zamri |
| author_facet | H., Plumeriastuti Saad, Mohd Zamri |
| author_sort | H., Plumeriastuti |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and subjected to RBPT for antibody titres. Uterii, mammary glands and supra-mammary lymph nodes were examined for pathological changes, immuno-peroxidase (IP) staining, bacterial isolation and PCR to detect B. melitensis. Results revealed that of the 27 goats tested, 41% were Category I (antibody titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4). Goats in Categories 2 and 3 revealed more severe pathological changes with the latter being most severe. All goats in Category 3 showed positive reaction to IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%) goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There was strong correlation between the antibody titre, pathological lesions and presence of B. melitensis. |
| first_indexed | 2025-11-15T08:44:42Z |
| format | Article |
| id | upm-25417 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:44:42Z |
| publishDate | 2012 |
| publisher | Online Journal of Veterinary Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-254172015-09-14T00:07:49Z http://psasir.upm.edu.my/id/eprint/25417/ Detection of Brucella melitensis in seropositive goats. H., Plumeriastuti Saad, Mohd Zamri This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and subjected to RBPT for antibody titres. Uterii, mammary glands and supra-mammary lymph nodes were examined for pathological changes, immuno-peroxidase (IP) staining, bacterial isolation and PCR to detect B. melitensis. Results revealed that of the 27 goats tested, 41% were Category I (antibody titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4). Goats in Categories 2 and 3 revealed more severe pathological changes with the latter being most severe. All goats in Category 3 showed positive reaction to IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%) goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There was strong correlation between the antibody titre, pathological lesions and presence of B. melitensis. Online Journal of Veterinary Research 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25417/1/Detection%20of%20Brucella%20melitensis%20in%20seropositive%20goats.pdf H., Plumeriastuti and Saad, Mohd Zamri (2012) Detection of Brucella melitensis in seropositive goats. Online Journal of Veterinary Research, 16 (1). pp. 1-7. ISSN 1328-925X English |
| spellingShingle | H., Plumeriastuti Saad, Mohd Zamri Detection of Brucella melitensis in seropositive goats. |
| title | Detection of Brucella melitensis in seropositive goats. |
| title_full | Detection of Brucella melitensis in seropositive goats. |
| title_fullStr | Detection of Brucella melitensis in seropositive goats. |
| title_full_unstemmed | Detection of Brucella melitensis in seropositive goats. |
| title_short | Detection of Brucella melitensis in seropositive goats. |
| title_sort | detection of brucella melitensis in seropositive goats. |
| url | http://psasir.upm.edu.my/id/eprint/25417/ http://psasir.upm.edu.my/id/eprint/25417/1/Detection%20of%20Brucella%20melitensis%20in%20seropositive%20goats.pdf |