Detection of Brucella melitensis in seropositive goats.
This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and sub...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Online Journal of Veterinary Research
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25417/ http://psasir.upm.edu.my/id/eprint/25417/1/Detection%20of%20Brucella%20melitensis%20in%20seropositive%20goats.pdf |
| Summary: | This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and subjected to RBPT for antibody titres. Uterii, mammary glands and supra-mammary lymph nodes were examined for pathological changes, immuno-peroxidase (IP) staining, bacterial isolation and PCR to detect B. melitensis. Results revealed that of the 27 goats tested, 41% were Category I (antibody titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4). Goats in Categories 2 and 3 revealed more severe pathological changes with the latter being most severe. All goats in Category 3 showed positive reaction to IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%) goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There was strong correlation between the antibody titre, pathological lesions and presence of B. melitensis. |
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