Rapid detection of pathogenic leptospires by lyophilized reagent-based Polymerase Chain Reaction.

QR Code

Rapid detection of pathogenic leptospires by lyophilized reagent-based Polymerase Chain Reaction.

A simple and reliable tool for the early diagnosis of leptospirosis is urgently needed. We report the development of a lyophilized reagent-based polymerase chain reaction (PCR) assay targeting lipL32 gene, which is present only in pathogenic leptospires. To determine the effectiveness of the newly d...

Full description

Bibliographic Details
Main Authors:	Lee, S.V., Tai, E.S., Mutalib, Abdul Rahim, Bejo, Siti Khairani, Bahaman, Abdul Rani
Format:	Article
Language:	English English
Published:	Malaysian Society of Parasitology and Tropical Medicine 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/25403/ http://psasir.upm.edu.my/id/eprint/25403/1/Rapid%20detection%20of%20pathogenic%20leptospires%20by%20lyophilized%20reagent.pdf

Similar Items

Detection of leptospires from experimentally infected hamsters by the polymerase chain reaction assay
by: A. Rashid, Fairuz Ridzlan, et al.
Published: (2012)

Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice
by: Omar, Sharina, et al.
Published: (2007)

Comparison of culture and polymerase chain reaction for the detection of Brucella melitensis in experimentally infected mice.
by: Bejo, Siti Khairani, et al.
Published: (2008)

Antigenic potential of a recombinant polyvalent DNA vaccine against pathogenic leptospiral infection
by: Zakaria, Zunita, et al.
Published: (2019)

The polymerase chain reaction
by: McPherson, M., et al.
Published: (1992)

Experimental infection of hamsters with a local leptospiral isolate from rats
by: Lim, Bee Chi, et al.
Published: (2010)

Serological prevalence of leptospiral infection in wild rats at the National Service Training Centres in Kelantan and Terengganu
by: Mohamed Hassan, S. N., et al.
Published: (2010)

Leptospiral infections in stray dogs in Malaysia
by: Abdul Mutalib, Abdul Rahim, et al.
Published: (2009)

Polymerase chain reaction analysis as a rapid screening test for diagnosis of fragile X syndrome
by: Phan CL,, et al.
Published: (2006)

Polymerase Chain Reaction Based-Assays for Rapid Detection and Subtyping Of Type a Influenza Viruses
by: Chaharaein, Broomand
Published: (2006)

Rapid detection of Newcastle disease virus (NDV) with SYBR Green I real-time polymerase chain reaction
by: Tan, Sheau Wei, et al.
Published: (2004)

The role of buffaloes in the epidemiology of leptospiral infection in Malaysia.
by: Bahaman, Abdul Rani, et al.
Published: (1983)

Room-temperature stable loop-mediated isothermal amplification LAMP reagents to detect leptospiral DNA
by: Lee, Pui Yuei, et al.
Published: (2021)

Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.
by: Bamaiyi , Pwaveno Huladeino, et al.
Published: (2011)

Characterization Of Leptospiral Isolates Obtained From Selected Cattle Farms In Malaysia
by: Bejo, Siti Khairani
Published: (1997)

Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2004)

The effect of variable taq Polymerase concentrations in Polymerase chain reaction (PCR) / Zanura Adzhari
by: Zanura, Adzhari
Published: (2007)

Serological prevalence of leptospiral infection in cattle from ladang angkat and Taman Pertanian Universiti, Universiti Putra Malaysia
by: Zulkapli, Azlina, et al.
Published: (2012)

Site-directed mutagenesis on plasmid using polymerase chain reaction
by: Ahmad, Abdul Aziz, et al.
Published: (2018)

Investigation of heat transfer effect on polymerase chain reaction
by: Kafafy, Raed Ismail
Published: (2012)

A Serological And Bacteriological Study Of Leptospiral Infection In Domestic Animals In Peninsular Malaysia
by: Bahaman, Abdul Rani
Published: (1988)

Enhancement of polymerase chain reaction using graphene nano-flakes
by: Rasheed, Abdul Khaliq, et al.
Published: (2011)

Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
by: Khaliq R., Abdul, et al.
Published: (2012)

Polymerase chain reaction optimization and polymerase chain reaction-restriction fragment length polymorphism of ambal riong (Solen sarawakensis) from bay area
by: Florence, Laiping
Published: (2007)

Multiplex touchdown polymerase chain reaction for rapid detection of Salmonella enterica subsp. enterica serovars Enteritidis and Typhimurium in food
by: Sandrasaigaran, P., et al.
Published: (2023)

Molecular detection of Leptospira sp. in cattle and goats in Kelantan, Malaysia after a massive flood using multiplex polymerase chain reaction
by: Sabri, A. R., et al.
Published: (2019)

Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
by: Abdullah Al-Haj, Nagi Ahmed, et al.
Published: (2009)

Diagnosis of neonatal meningitis: is it time to use polymerase chain reaction?
by: Zainudin, Zurina, et al.
Published: (2021)

Detection of S. Enteritidis in artificially-contaminated raw chicken meat by polymerase chain reaction
by: Abdul Talib, Mohd Afendy, et al.
Published: (2012)

Experimental trials on the use of radioimmunoassay for the detection of leptospiral antigens in urine
by: Bahaman, Abdul Rani, et al.
Published: (1986)

Detection of infectious bursal disease virus using SYBR Green 1 based realtime polymerase chain reaction
by: Hamzah, Hairul Aini, et al.
Published: (2004)

Detection of Citrus Greening Organism (Liberobacter Asiaticum) by Polymerase Chain Reaction
by: Mahmud Sara, Muh. Asaad
Published: (2001)

Detection of Glasser's Disease in clinical samples using Polymerase chain reaction.
by: Teh, See Wai, et al.
Published: (2011)

Detection of avian leukosis virus using polymerase chain reaction and enzyme linked immunosorbent assay
by: Thapa Chhetri, Bala Ram, et al.
Published: (2004)

Porcine specific real-time polymerase chain reaction (PCR) for halal verification
by: Khalid, Farihah Liyana, et al.
Published: (2009)

Risk factors and prediction of leptospiral seropositivity among dogs and dog handlers in Malaysia
by: Goh, Soon Heng, et al.
Published: (2019)

Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation
by: Abdullah, Faez Firdaus Jesse, et al.
Published: (2013)

Polymerase chain reaction detection of Corynebacterium pseudotuberculosis in mice following oral inoculation with the bacteria
by: Muhdi, Ahmad Fauzan, et al.
Published: (2012)

On-site rapid detection of toxic Alexandrium tamiyavanichii: integrating the species-specific hydrolysis probe in insulated isothermal polymerase chain reaction (iiPCR)
by: Nyuk, Fong Kon, et al.
Published: (2016)

Polymerase chain reaction-based discrimination of viable from non-viable Mycoplasma gallisepticum
by: Tan, Ching Giap, et al.
Published: (2014)