Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.

Neopolystoma liewi Du Preez and Lim, 2000, is redescribed on the basis of specimens collected from the type host, Cuora amboinensis, in Malaysia during 2008. Morphometric comparison of the new specimens with the original description revealed significant differences because of differences in specim...

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Main Authors: Platt, Thomas R., Firth, Andrea, Sharma, Reuben Sunil Kumar
Format: Article
Language:English
Published: Helminthological Society of Washington 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25360/
http://psasir.upm.edu.my/id/eprint/25360/1/Redescription%20of%20neopolystoma%20liewi%20Du%20Preez%20and%20Lim.pdf
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author Platt, Thomas R.
Firth, Andrea
Sharma, Reuben Sunil Kumar
author_facet Platt, Thomas R.
Firth, Andrea
Sharma, Reuben Sunil Kumar
author_sort Platt, Thomas R.
building UPM Institutional Repository
collection Online Access
description Neopolystoma liewi Du Preez and Lim, 2000, is redescribed on the basis of specimens collected from the type host, Cuora amboinensis, in Malaysia during 2008. Morphometric comparison of the new specimens with the original description revealed significant differences because of differences in specimen preparation. The current material was killed in hot formalin without coverglass pressure, whereas the specimens from the original description were killed and fixed with coverglass pressure. Coverglass pressure resulted in increases of 30%–70% in most measurements of body organs. Sclerotized structures (genital and haptoral hooks and eggs) or structur es with sclerotized supports (haptoral suckers) were not affected by coverglass pressure. The coefficient of variation of measurements from worms killed with hot formalin was low (,10%) for most structures. The authors recommend discontinuing the use of flattening in the preparation of monogenetic trematodes, with the exception of a small number of specimens necessary to accurately view structures not easily seen in unflattened material. Measurements from flattened specimens should not be used in species descriptions.
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spelling upm-253602015-10-28T08:48:48Z http://psasir.upm.edu.my/id/eprint/25360/ Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation. Platt, Thomas R. Firth, Andrea Sharma, Reuben Sunil Kumar Neopolystoma liewi Du Preez and Lim, 2000, is redescribed on the basis of specimens collected from the type host, Cuora amboinensis, in Malaysia during 2008. Morphometric comparison of the new specimens with the original description revealed significant differences because of differences in specimen preparation. The current material was killed in hot formalin without coverglass pressure, whereas the specimens from the original description were killed and fixed with coverglass pressure. Coverglass pressure resulted in increases of 30%–70% in most measurements of body organs. Sclerotized structures (genital and haptoral hooks and eggs) or structur es with sclerotized supports (haptoral suckers) were not affected by coverglass pressure. The coefficient of variation of measurements from worms killed with hot formalin was low (,10%) for most structures. The authors recommend discontinuing the use of flattening in the preparation of monogenetic trematodes, with the exception of a small number of specimens necessary to accurately view structures not easily seen in unflattened material. Measurements from flattened specimens should not be used in species descriptions. Helminthological Society of Washington 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25360/1/Redescription%20of%20neopolystoma%20liewi%20Du%20Preez%20and%20Lim.pdf Platt, Thomas R. and Firth, Andrea and Sharma, Reuben Sunil Kumar (2011) Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation. Comparative Parasitology, 78 (2). pp. 286-290. ISSN 1525-2647 10.1654/4514.1
spellingShingle Platt, Thomas R.
Firth, Andrea
Sharma, Reuben Sunil Kumar
Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title_full Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title_fullStr Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title_full_unstemmed Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title_short Redescription of neopolystoma liewi Du Preez and Lim, 2000 (Monogenea: Polystomatidae) from Cuora amboinensis (Testudines: Geomydidae) with notes on specimen preparation.
title_sort redescription of neopolystoma liewi du preez and lim, 2000 (monogenea: polystomatidae) from cuora amboinensis (testudines: geomydidae) with notes on specimen preparation.
url http://psasir.upm.edu.my/id/eprint/25360/
http://psasir.upm.edu.my/id/eprint/25360/
http://psasir.upm.edu.my/id/eprint/25360/1/Redescription%20of%20neopolystoma%20liewi%20Du%20Preez%20and%20Lim.pdf