Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein.
Despite all efforts, there is still no effective vaccine available against the human respiratory syncytial virus (HRSV) that is a major cause of severe lower respiratory tract disease in infants and the elderly. In this review, we examined the potential of the conserved immunogenic region (residues...
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| Language: | English English |
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Wiley-Blackwell
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25358/ http://psasir.upm.edu.my/id/eprint/25358/1/Dynamics%20of%20the%20bacterially%20expressed%20conserved%20immunogenic%20region%20of%20the%20human%20respiratory%20syncytial%20virus%20G%20protein.pdf |
| _version_ | 1848845288495644672 |
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| author | Jalilian, Farid Azizi Jahanshiri, Fatemeh Sekawi, Zamberi Omar, Abdul Rahman Yusoff, Khatijah |
| author_facet | Jalilian, Farid Azizi Jahanshiri, Fatemeh Sekawi, Zamberi Omar, Abdul Rahman Yusoff, Khatijah |
| author_sort | Jalilian, Farid Azizi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Despite all efforts, there is still no effective vaccine available against the human respiratory syncytial virus (HRSV) that is a major cause of severe lower respiratory tract disease in infants and the elderly. In this review, we examined the potential of the conserved immunogenic region (residues 122-226) of the HRSV glycoprotein G alone as the inducer of neutralizing antibodies against this virus. The Escherichia coli produced recombinant conserved region of G (designated as G domain), which was used for rabbit immunization. Although rabbit is a semipermissive host for HRSV, our result showed that the polyclonal antibodies against the G domain protein could strongly neutralize the virus (69.3%), suggesting that the G immunogenic region of HRSV alone has a great potential in vaccine development. To our knowledge, this is the first report in which neutralizing antibodies to respiratory syncytial virus have been evoked using bacterially expressed G immunogenic domain protein without any adjuvant. |
| first_indexed | 2025-11-15T08:44:26Z |
| format | Article |
| id | upm-25358 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:44:26Z |
| publishDate | 2011 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-253582015-10-06T06:32:22Z http://psasir.upm.edu.my/id/eprint/25358/ Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. Jalilian, Farid Azizi Jahanshiri, Fatemeh Sekawi, Zamberi Omar, Abdul Rahman Yusoff, Khatijah Despite all efforts, there is still no effective vaccine available against the human respiratory syncytial virus (HRSV) that is a major cause of severe lower respiratory tract disease in infants and the elderly. In this review, we examined the potential of the conserved immunogenic region (residues 122-226) of the HRSV glycoprotein G alone as the inducer of neutralizing antibodies against this virus. The Escherichia coli produced recombinant conserved region of G (designated as G domain), which was used for rabbit immunization. Although rabbit is a semipermissive host for HRSV, our result showed that the polyclonal antibodies against the G domain protein could strongly neutralize the virus (69.3%), suggesting that the G immunogenic region of HRSV alone has a great potential in vaccine development. To our knowledge, this is the first report in which neutralizing antibodies to respiratory syncytial virus have been evoked using bacterially expressed G immunogenic domain protein without any adjuvant. Wiley-Blackwell 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25358/1/Dynamics%20of%20the%20bacterially%20expressed%20conserved%20immunogenic%20region%20of%20the%20human%20respiratory%20syncytial%20virus%20G%20protein.pdf Jalilian, Farid Azizi and Jahanshiri, Fatemeh and Sekawi, Zamberi and Omar, Abdul Rahman and Yusoff, Khatijah (2011) Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. Biotechnology and Applied Biochemistry, 58 (2 ). pp. 97-102. ISSN 0885-4513 http://onlinelibrary.wiley.com/ 10.1002/bab.19 English |
| spellingShingle | Jalilian, Farid Azizi Jahanshiri, Fatemeh Sekawi, Zamberi Omar, Abdul Rahman Yusoff, Khatijah Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title | Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title_full | Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title_fullStr | Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title_full_unstemmed | Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title_short | Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein. |
| title_sort | dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus g protein. |
| url | http://psasir.upm.edu.my/id/eprint/25358/ http://psasir.upm.edu.my/id/eprint/25358/ http://psasir.upm.edu.my/id/eprint/25358/ http://psasir.upm.edu.my/id/eprint/25358/1/Dynamics%20of%20the%20bacterially%20expressed%20conserved%20immunogenic%20region%20of%20the%20human%20respiratory%20syncytial%20virus%20G%20protein.pdf |