Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP...
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| Format: | Article |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25331/ http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf |
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| author | Moeini, Hassan Abdul Rahim, Raha Omar, Abdul Rahman Yusoff, Khatijah |
| author_facet | Moeini, Hassan Abdul Rahim, Raha Omar, Abdul Rahman Yusoff, Khatijah |
| author_sort | Moeini, Hassan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV. |
| first_indexed | 2025-11-15T08:44:18Z |
| format | Article |
| id | upm-25331 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:44:18Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
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| spelling | upm-253312015-10-16T07:13:23Z http://psasir.upm.edu.my/id/eprint/25331/ Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. Moeini, Hassan Abdul Rahim, Raha Omar, Abdul Rahman Yusoff, Khatijah In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV. Elsevier 2011-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf Moeini, Hassan and Abdul Rahim, Raha and Omar, Abdul Rahman and Yusoff, Khatijah (2011) Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. Comparative Immunology, Microbiology and Infectious Diseases, 34 (3). pp. 227-236. ISSN 0147-9571 http://www.elsevier.com/ 10.1016/j.cimid.2010.11.006 English |
| spellingShingle | Moeini, Hassan Abdul Rahim, Raha Omar, Abdul Rahman Yusoff, Khatijah Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title | Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title_full | Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title_fullStr | Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title_full_unstemmed | Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title_short | Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. |
| title_sort | development of a dna vaccine against chicken anemia virus by using a bicistronic vector expressing vp1 and vp2 proteins of cav. |
| url | http://psasir.upm.edu.my/id/eprint/25331/ http://psasir.upm.edu.my/id/eprint/25331/ http://psasir.upm.edu.my/id/eprint/25331/ http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf |