Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus
An initial study on gene cloning and characterization of unicellular green microalga Ankistrodesmus convolutus was carried out to isolate and characterize the full-length cDNA of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) as a first step towards elucidating the structure of...
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| Format: | Article |
| Language: | English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25180/ http://psasir.upm.edu.my/id/eprint/25180/1/Cloning%20and%20characterization%20of%20ribulose.pdf |
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| author | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Napis, Suhaimi |
| author_facet | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Napis, Suhaimi |
| author_sort | Thanh, Tran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An initial study on gene cloning and characterization of unicellular green microalga Ankistrodesmus convolutus was carried out to isolate and characterize the full-length cDNA of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) as a first step towards elucidating the structure of A. convolutus RbcS gene. The full-length of A. convolutus RbcS cDNA (AcRbcS) contained 28 bp of 5' untranslated region (UTR), 225 bp of 3' non-coding region, and an open reading frame of 165 amino acids consisting of a chloroplast transit peptide with 24 amino acids and a mature protein of 141 amino acids. The amino acid sequence has high identity to those of other green algae RbcS genes. The AcRbcS contained a few conserved domains including protein kinase C phosphorylation site, tyrosine kinase phosphorylation site and N-myristoylation sites. The AcRbcS was successfully expressed in Escherichia coli and a ~21 kDa of anticipated protein band was observed on SDS-PAGE. From the phylogenetic analysis of RbcS protein sequences, it was found that the RbcS of A. convolutus has closer genetic relationship with green microalgae species compared to those of green seaweed and green macroalgae species. Southern hybridization analysis revealed that the AcRbcS is a member of a small multigene family comprising of two to six members in A. convolutus genome. Under different illumination conditions, RT-PCR analysis showed that AcRbcS transcription was reduced in the dark, and drastically recovered in the light condition. Results presented in this paper established a good foundation for further study on the photosynthetic process of A. convolutus and other green algae species where little information is known on Rubisco small subunit. |
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| format | Article |
| id | upm-25180 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:43:39Z |
| publishDate | 2011 |
| publisher | Springer |
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| spelling | upm-251802017-09-28T07:54:24Z http://psasir.upm.edu.my/id/eprint/25180/ Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Napis, Suhaimi An initial study on gene cloning and characterization of unicellular green microalga Ankistrodesmus convolutus was carried out to isolate and characterize the full-length cDNA of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) as a first step towards elucidating the structure of A. convolutus RbcS gene. The full-length of A. convolutus RbcS cDNA (AcRbcS) contained 28 bp of 5' untranslated region (UTR), 225 bp of 3' non-coding region, and an open reading frame of 165 amino acids consisting of a chloroplast transit peptide with 24 amino acids and a mature protein of 141 amino acids. The amino acid sequence has high identity to those of other green algae RbcS genes. The AcRbcS contained a few conserved domains including protein kinase C phosphorylation site, tyrosine kinase phosphorylation site and N-myristoylation sites. The AcRbcS was successfully expressed in Escherichia coli and a ~21 kDa of anticipated protein band was observed on SDS-PAGE. From the phylogenetic analysis of RbcS protein sequences, it was found that the RbcS of A. convolutus has closer genetic relationship with green microalgae species compared to those of green seaweed and green macroalgae species. Southern hybridization analysis revealed that the AcRbcS is a member of a small multigene family comprising of two to six members in A. convolutus genome. Under different illumination conditions, RT-PCR analysis showed that AcRbcS transcription was reduced in the dark, and drastically recovered in the light condition. Results presented in this paper established a good foundation for further study on the photosynthetic process of A. convolutus and other green algae species where little information is known on Rubisco small subunit. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25180/1/Cloning%20and%20characterization%20of%20ribulose.pdf Thanh, Tran and Chi, Vu Thi Quynh and Abdullah, Mohd Puad and Omar, Hishamuddin and Noroozi, Mostafa and Napis, Suhaimi (2011) Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus. Molecular Biology Reports, 38 (8). pp. 5297-5305. ISSN 0301-4851; ESSN: 1573-4978 https://rd.springer.com/article/10.1007%2Fs11033-011-0679-4 10.1007/s11033-011-0679-4 |
| spellingShingle | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Napis, Suhaimi Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title | Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title_full | Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title_fullStr | Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title_full_unstemmed | Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title_short | Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus |
| title_sort | cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcs) cdna from green microalga ankistrodesmus convolutus |
| url | http://psasir.upm.edu.my/id/eprint/25180/ http://psasir.upm.edu.my/id/eprint/25180/ http://psasir.upm.edu.my/id/eprint/25180/ http://psasir.upm.edu.my/id/eprint/25180/1/Cloning%20and%20characterization%20of%20ribulose.pdf |