Cytotoxic constituents of Clausena excavata

Phytochemical investigation on leaves, stem bark and roots of Malaysian Clausena excavata has led to the isolation and identification of limonoid compounds, clausenolide-1-methyl ether (1) and clausenarin (2), carbazole alkaloids, 3-formyl-2,7-dimethoxycarbazole (3) and clausine-K (4) together with...

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Main Authors: Mohd Shariff, Nurul Waznah, Mustahil, Noorul Adawiyah, Mohd Noor, Halimatul Saadiah, Md. Sukari, Mohd Aspollah, Rahmani, Mawardi, Yap, Taufiq Yun Hin, Ee, Gwendoline Cheng Lian
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25062/
http://psasir.upm.edu.my/id/eprint/25062/1/25062.pdf
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author Mohd Shariff, Nurul Waznah
Mustahil, Noorul Adawiyah
Mohd Noor, Halimatul Saadiah
Md. Sukari, Mohd Aspollah
Rahmani, Mawardi
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
author_facet Mohd Shariff, Nurul Waznah
Mustahil, Noorul Adawiyah
Mohd Noor, Halimatul Saadiah
Md. Sukari, Mohd Aspollah
Rahmani, Mawardi
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
author_sort Mohd Shariff, Nurul Waznah
building UPM Institutional Repository
collection Online Access
description Phytochemical investigation on leaves, stem bark and roots of Malaysian Clausena excavata has led to the isolation and identification of limonoid compounds, clausenolide-1-methyl ether (1) and clausenarin (2), carbazole alkaloids, 3-formyl-2,7-dimethoxycarbazole (3) and clausine-K (4) together with coumarins, xanthyletin (5), dentatin (6) and nordentatin (7). Extracts of roots and isolated compounds (1), (2), (5) and (6) were subjected to cytotoxic screening against various cancer cell lines (HL-60, MCF- 7, HeLa and HT-29). All roots extracts except methanol showed strong activity against HL-60 and MCF-7 cancer cell lines with IC 50 values ranging from 4 to 6 μg/ml. Dentatin (6) was found to be the most cytotoxic constituent against all cancer cell lines with IC50 values ranging from 5 to 10 μg/ml.
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spelling upm-250622017-11-15T03:38:28Z http://psasir.upm.edu.my/id/eprint/25062/ Cytotoxic constituents of Clausena excavata Mohd Shariff, Nurul Waznah Mustahil, Noorul Adawiyah Mohd Noor, Halimatul Saadiah Md. Sukari, Mohd Aspollah Rahmani, Mawardi Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Phytochemical investigation on leaves, stem bark and roots of Malaysian Clausena excavata has led to the isolation and identification of limonoid compounds, clausenolide-1-methyl ether (1) and clausenarin (2), carbazole alkaloids, 3-formyl-2,7-dimethoxycarbazole (3) and clausine-K (4) together with coumarins, xanthyletin (5), dentatin (6) and nordentatin (7). Extracts of roots and isolated compounds (1), (2), (5) and (6) were subjected to cytotoxic screening against various cancer cell lines (HL-60, MCF- 7, HeLa and HT-29). All roots extracts except methanol showed strong activity against HL-60 and MCF-7 cancer cell lines with IC 50 values ranging from 4 to 6 μg/ml. Dentatin (6) was found to be the most cytotoxic constituent against all cancer cell lines with IC50 values ranging from 5 to 10 μg/ml. Academic Journals 2011-11-16 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25062/1/25062.pdf Mohd Shariff, Nurul Waznah and Mustahil, Noorul Adawiyah and Mohd Noor, Halimatul Saadiah and Md. Sukari, Mohd Aspollah and Rahmani, Mawardi and Yap, Taufiq Yun Hin and Ee, Gwendoline Cheng Lian (2011) Cytotoxic constituents of Clausena excavata. African Journal of Biotechnology, 10 (72). art. no. 5CCFF9731856. pp. 16337-16341. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/5CCFF9731856 10.5897/AJB11.2239
spellingShingle Mohd Shariff, Nurul Waznah
Mustahil, Noorul Adawiyah
Mohd Noor, Halimatul Saadiah
Md. Sukari, Mohd Aspollah
Rahmani, Mawardi
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
Cytotoxic constituents of Clausena excavata
title Cytotoxic constituents of Clausena excavata
title_full Cytotoxic constituents of Clausena excavata
title_fullStr Cytotoxic constituents of Clausena excavata
title_full_unstemmed Cytotoxic constituents of Clausena excavata
title_short Cytotoxic constituents of Clausena excavata
title_sort cytotoxic constituents of clausena excavata
url http://psasir.upm.edu.my/id/eprint/25062/
http://psasir.upm.edu.my/id/eprint/25062/
http://psasir.upm.edu.my/id/eprint/25062/
http://psasir.upm.edu.my/id/eprint/25062/1/25062.pdf