Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers

Koompassia malaccensis (Leguminosae), locally known as kempas, is an important tropical timber species in South-East Asia. Although studies have shown that most tropical tree species are predominantly outcrossing, there is no empirical support for this species prior to this study, with regard to its...

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Main Authors: Lee, Chai Ting, Lee, Soon Leong, Ng, Kevin Kit Siong, Qamaruz Zaman, Faridah, Siraj, Siti Shapor, Muhammad, Norwati
Format: Article
Language:English
Published: Forest Research Institute Malaysia 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24985/
http://psasir.upm.edu.my/id/eprint/24985/1/Estimation%20of%20outcrossing%20rates%20in%20koompassia%20malaccensts%20from%20an%20open.pdf
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author Lee, Chai Ting
Lee, Soon Leong
Ng, Kevin Kit Siong
Qamaruz Zaman, Faridah
Siraj, Siti Shapor
Muhammad, Norwati
author_facet Lee, Chai Ting
Lee, Soon Leong
Ng, Kevin Kit Siong
Qamaruz Zaman, Faridah
Siraj, Siti Shapor
Muhammad, Norwati
author_sort Lee, Chai Ting
building UPM Institutional Repository
collection Online Access
description Koompassia malaccensis (Leguminosae), locally known as kempas, is an important tropical timber species in South-East Asia. Although studies have shown that most tropical tree species are predominantly outcrossing, there is no empirical support for this species prior to this study, with regard to its mating system. Information on its reproductive biology is also scanty. We report the estimation of the outcrossing rates of K. malaccensis using microsatellite markers, based on a fruiting season at the Semangkok Forest Reserve, Selangor. Microsatellite analysis was performed for an average of 46 seeds each from nine adult K. malaccensis trees, using four polymorphic microsatellite loci (Kma050, Kma067, Kma147 and Kma180). Single and multilocus population outcrossing estimates (ts and tm respectively) were determined using the software MLTR version 3.0. Results showed that this timber species was predominantly outcrossing (tm = 0.890). Biparental mating (tm – ts) was very low, only 0.026, suggesting low tendency of mating between relatives. Outcrossing estimates obtained for individual mother trees were in the range of 0.637 to 0.994. The relatively lower outcrossing rates exhibited by a few progeny arrays indicated that K. malaccensis was not completely self-incompatible.
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spelling upm-249852019-10-30T00:49:21Z http://psasir.upm.edu.my/id/eprint/24985/ Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers Lee, Chai Ting Lee, Soon Leong Ng, Kevin Kit Siong Qamaruz Zaman, Faridah Siraj, Siti Shapor Muhammad, Norwati Koompassia malaccensis (Leguminosae), locally known as kempas, is an important tropical timber species in South-East Asia. Although studies have shown that most tropical tree species are predominantly outcrossing, there is no empirical support for this species prior to this study, with regard to its mating system. Information on its reproductive biology is also scanty. We report the estimation of the outcrossing rates of K. malaccensis using microsatellite markers, based on a fruiting season at the Semangkok Forest Reserve, Selangor. Microsatellite analysis was performed for an average of 46 seeds each from nine adult K. malaccensis trees, using four polymorphic microsatellite loci (Kma050, Kma067, Kma147 and Kma180). Single and multilocus population outcrossing estimates (ts and tm respectively) were determined using the software MLTR version 3.0. Results showed that this timber species was predominantly outcrossing (tm = 0.890). Biparental mating (tm – ts) was very low, only 0.026, suggesting low tendency of mating between relatives. Outcrossing estimates obtained for individual mother trees were in the range of 0.637 to 0.994. The relatively lower outcrossing rates exhibited by a few progeny arrays indicated that K. malaccensis was not completely self-incompatible. Forest Research Institute Malaysia 2011-10-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24985/1/Estimation%20of%20outcrossing%20rates%20in%20koompassia%20malaccensts%20from%20an%20open.pdf Lee, Chai Ting and Lee, Soon Leong and Ng, Kevin Kit Siong and Qamaruz Zaman, Faridah and Siraj, Siti Shapor and Muhammad, Norwati (2011) Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers. Journal of Tropical Forest Science, 23 (4). pp. 410-416. ISSN 0128-1283 https://www.frim.gov.my/publication/journal-of-tropical-forest-science-jtfs/
spellingShingle Lee, Chai Ting
Lee, Soon Leong
Ng, Kevin Kit Siong
Qamaruz Zaman, Faridah
Siraj, Siti Shapor
Muhammad, Norwati
Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title_full Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title_fullStr Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title_full_unstemmed Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title_short Estimation of outcrossing rates in Koompassia malaccensts from an open-pollinated population in Peninsular Malaysia using microsatellite markers
title_sort estimation of outcrossing rates in koompassia malaccensts from an open-pollinated population in peninsular malaysia using microsatellite markers
url http://psasir.upm.edu.my/id/eprint/24985/
http://psasir.upm.edu.my/id/eprint/24985/
http://psasir.upm.edu.my/id/eprint/24985/1/Estimation%20of%20outcrossing%20rates%20in%20koompassia%20malaccensts%20from%20an%20open.pdf