Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis.
Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of bro...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Ribeirão Preto foundation for Scientific Research (FUNPEC)
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24748/ http://psasir.upm.edu.my/id/eprint/24748/1/Food%20assimilated%20by%20two%20sympatric%20populations%20of%20the%20brown%20planthopper%20Nilaparvata%20lugens.pdf |
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| author | Latif, M.A. Md Yusoh, Omar Tan, Soon Guan Siraj, Siti Shapor Ali, M.E. Yusop, Mohd Rafii |
| author_facet | Latif, M.A. Md Yusoh, Omar Tan, Soon Guan Siraj, Siti Shapor Ali, M.E. Yusop, Mohd Rafii |
| author_sort | Latif, M.A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates that of herbivore insects in extractions of whole insects. Ingestion and assimilation of food were reduced significantly when individuals derived from one host plant were caged on the other species. The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35), pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in both rice-infesting populations of brown planthopper and its host plant (rice). Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3 (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant. So, it is clear that the DNA bands amplified in the host plants were also found in the extracts from the insects feeding on them. |
| first_indexed | 2025-11-15T08:41:46Z |
| format | Article |
| id | upm-24748 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:41:46Z |
| publishDate | 2012 |
| publisher | Ribeirão Preto foundation for Scientific Research (FUNPEC) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-247482015-10-02T03:11:00Z http://psasir.upm.edu.my/id/eprint/24748/ Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. Latif, M.A. Md Yusoh, Omar Tan, Soon Guan Siraj, Siti Shapor Ali, M.E. Yusop, Mohd Rafii Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates that of herbivore insects in extractions of whole insects. Ingestion and assimilation of food were reduced significantly when individuals derived from one host plant were caged on the other species. The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35), pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in both rice-infesting populations of brown planthopper and its host plant (rice). Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3 (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant. So, it is clear that the DNA bands amplified in the host plants were also found in the extracts from the insects feeding on them. Ribeirão Preto foundation for Scientific Research (FUNPEC) 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24748/1/Food%20assimilated%20by%20two%20sympatric%20populations%20of%20the%20brown%20planthopper%20Nilaparvata%20lugens.pdf Latif, M.A. and Md Yusoh, Omar and Tan, Soon Guan and Siraj, Siti Shapor and Ali, M.E. and Yusop, Mohd Rafii (2012) Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. Genetics and Molecular Research, 11 (1). pp. 30-41. ISSN 1676-5680; ESSN:1676-5680 http://www.jdb.uzh.ch/ 10.4238/2012.January.9.4 English |
| spellingShingle | Latif, M.A. Md Yusoh, Omar Tan, Soon Guan Siraj, Siti Shapor Ali, M.E. Yusop, Mohd Rafii Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title | Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title_full | Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title_fullStr | Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title_full_unstemmed | Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title_short | Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis. |
| title_sort | food assimilated by two sympatric populations of the brown planthopper nilaparvata lugens (delphacidae) feeding on different host plants contaminates insect dna detected by rapd-pcr analysis. |
| url | http://psasir.upm.edu.my/id/eprint/24748/ http://psasir.upm.edu.my/id/eprint/24748/ http://psasir.upm.edu.my/id/eprint/24748/ http://psasir.upm.edu.my/id/eprint/24748/1/Food%20assimilated%20by%20two%20sympatric%20populations%20of%20the%20brown%20planthopper%20Nilaparvata%20lugens.pdf |