Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran.
Objectives of this study were to investigate the prevalence of K. pneumoniae producing ESBLs, to evaluate the susceptibility of K. pneumoniae producing ESBLs towards non-beta-lactam antibiotics and to study the dominant ESBLs gene in Emam Reza hospital. K. pneumoniae producing ESBLs identified by ph...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Oriental Scientific Publishing Co.
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24737/ http://psasir.upm.edu.my/id/eprint/24737/1/Phenotypic%20and%20genotypic%20assay%20for%20detection%20of%20extended%20spectrum%20B.pdf |
| _version_ | 1848845118234165248 |
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| author | Ghafourian, Sobhan Sadeghifard, Nourkhoda Sekawi, Zamberi Neela, Vasante Kumari Shamsudin, Mariana Nor Pakzad, Iraj Galehdari, Elham Abouali Maleki, Abbas Pornour, Majid Mobaiyen, Haedeh Rahbar, Mohammad |
| author_facet | Ghafourian, Sobhan Sadeghifard, Nourkhoda Sekawi, Zamberi Neela, Vasante Kumari Shamsudin, Mariana Nor Pakzad, Iraj Galehdari, Elham Abouali Maleki, Abbas Pornour, Majid Mobaiyen, Haedeh Rahbar, Mohammad |
| author_sort | Ghafourian, Sobhan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objectives of this study were to investigate the prevalence of K. pneumoniae producing ESBLs, to evaluate the susceptibility of K. pneumoniae producing ESBLs towards non-beta-lactam antibiotics and to study the dominant ESBLs gene in Emam Reza hospital. K. pneumoniae producing ESBLs identified by phenotypic and genotypic methods. Polymerase Chain Reaction (PCR) performed for detection of blaSHV, TEM and CTX-M. The findings showed that 43.69%, 13.59%, 7.77%, 11.65% and 23.3% were from UTI, ICUs, surgery ward, lesion infections and RTI, respectively. The results showed that
43.7% of isolates were ESBLs produces. The findings revealed that 26.7%, 6.7%, 20% and 0% of K.pneumoniae producing ESBLs were resistant to amikacin, ciprofloxacin,
cotrimoxazol and imipenem, respectively. Thirty-nine blaSHV, seven blaTEM and seven blaCTX-M identified among K.pneumoniae producing ESBLs. The results reflected in
cold month resistant to third generation cephalosporins were more than warm months. Generally, frequency of blaSHV was more than blaCTX-M and blaTEM. |
| first_indexed | 2025-11-15T08:41:44Z |
| format | Article |
| id | upm-24737 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:41:44Z |
| publishDate | 2011 |
| publisher | Oriental Scientific Publishing Co. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-247372015-11-26T08:43:54Z http://psasir.upm.edu.my/id/eprint/24737/ Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. Ghafourian, Sobhan Sadeghifard, Nourkhoda Sekawi, Zamberi Neela, Vasante Kumari Shamsudin, Mariana Nor Pakzad, Iraj Galehdari, Elham Abouali Maleki, Abbas Pornour, Majid Mobaiyen, Haedeh Rahbar, Mohammad Objectives of this study were to investigate the prevalence of K. pneumoniae producing ESBLs, to evaluate the susceptibility of K. pneumoniae producing ESBLs towards non-beta-lactam antibiotics and to study the dominant ESBLs gene in Emam Reza hospital. K. pneumoniae producing ESBLs identified by phenotypic and genotypic methods. Polymerase Chain Reaction (PCR) performed for detection of blaSHV, TEM and CTX-M. The findings showed that 43.69%, 13.59%, 7.77%, 11.65% and 23.3% were from UTI, ICUs, surgery ward, lesion infections and RTI, respectively. The results showed that 43.7% of isolates were ESBLs produces. The findings revealed that 26.7%, 6.7%, 20% and 0% of K.pneumoniae producing ESBLs were resistant to amikacin, ciprofloxacin, cotrimoxazol and imipenem, respectively. Thirty-nine blaSHV, seven blaTEM and seven blaCTX-M identified among K.pneumoniae producing ESBLs. The results reflected in cold month resistant to third generation cephalosporins were more than warm months. Generally, frequency of blaSHV was more than blaCTX-M and blaTEM. Oriental Scientific Publishing Co. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24737/1/Phenotypic%20and%20genotypic%20assay%20for%20detection%20of%20extended%20spectrum%20B.pdf Ghafourian, Sobhan and Sadeghifard, Nourkhoda and Sekawi, Zamberi and Neela, Vasante Kumari and Shamsudin, Mariana Nor and Pakzad, Iraj and Galehdari, Elham Abouali and Maleki, Abbas and Pornour, Majid and Mobaiyen, Haedeh and Rahbar, Mohammad (2011) Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. Journal of Pure and Applied Microbiology, 5 (1). pp. 1-10. ISSN 0973-7510 English |
| spellingShingle | Ghafourian, Sobhan Sadeghifard, Nourkhoda Sekawi, Zamberi Neela, Vasante Kumari Shamsudin, Mariana Nor Pakzad, Iraj Galehdari, Elham Abouali Maleki, Abbas Pornour, Majid Mobaiyen, Haedeh Rahbar, Mohammad Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title | Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title_full | Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title_fullStr | Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title_full_unstemmed | Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title_short | Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran. |
| title_sort | phenotypic and genotypic assay for detection of extended spectrum b-lactamases production by klebsiella pnemoniae isolates in emam reza hospital in tabriz, iran. |
| url | http://psasir.upm.edu.my/id/eprint/24737/ http://psasir.upm.edu.my/id/eprint/24737/1/Phenotypic%20and%20genotypic%20assay%20for%20detection%20of%20extended%20spectrum%20B.pdf |