Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein
Thymoquinone (TQ), the active constituent of Nigella sativa or black cumin exhibited cytotoxic effects in several cancer cell lines. In this study, the cytotoxicity of TQ in human cervical squamous carcinoma cells (SiHa) was investigated. TQ was cytotoxic towards SiHa cells with IC50 values of 10.67...
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24708/ http://psasir.upm.edu.my/id/eprint/24708/1/Thmoquinone%20from%20Nigella%20sativa%20was%20more%20potent%20than%20cisplatin%20in%20eliminating%20of%20SiHa%20cells%20via%20apoptosis%20with%20down.pdf |
| _version_ | 1848845110148595712 |
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| author | Ng, Wei Keat Saiful Yazan, Latifah Ismail, Maznah |
| author_facet | Ng, Wei Keat Saiful Yazan, Latifah Ismail, Maznah |
| author_sort | Ng, Wei Keat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thymoquinone (TQ), the active constituent of Nigella sativa or black cumin exhibited cytotoxic effects in several cancer cell lines. In this study, the cytotoxicity of TQ in human cervical squamous carcinoma cells (SiHa) was investigated. TQ was cytotoxic towards SiHa cells with IC50 values of 10.67 ± 0.12 and 9.33 ± 0.19 μg/mL as determined by MTT assay and trypan blue dye exclusion test, respectively, after 72 h of incubation. TQ was more cytotoxic towards SiHa cells compared to cisplatin. Interestingly, TQ was less cytotoxic towards the normal cells (3T3-L1 and Vero). Cell cycle analysis performed by flowcytometer showed a significant increase in the accumulation of TQ-treated cells at sub-G1 phase, indicating induction of apoptosis by the compound. Apoptosis induction by TQ was further confirmed by Annexin V/PI and AO/PI staining. Significant elevation of p53 and down-regulation of the anti-apoptotic Bcl-2 protein was found in the treated cells, without any changes in the expression of the pro-apoptotic Bax protein. In conclusion, thymoquinone from N. sativa was more potent than cisplatin in elimination of SiHa cells via apoptosis with down-regulation of Bcl-2 protein. |
| first_indexed | 2025-11-15T08:41:36Z |
| format | Article |
| id | upm-24708 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:41:36Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-247082018-04-09T09:21:51Z http://psasir.upm.edu.my/id/eprint/24708/ Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein Ng, Wei Keat Saiful Yazan, Latifah Ismail, Maznah Thymoquinone (TQ), the active constituent of Nigella sativa or black cumin exhibited cytotoxic effects in several cancer cell lines. In this study, the cytotoxicity of TQ in human cervical squamous carcinoma cells (SiHa) was investigated. TQ was cytotoxic towards SiHa cells with IC50 values of 10.67 ± 0.12 and 9.33 ± 0.19 μg/mL as determined by MTT assay and trypan blue dye exclusion test, respectively, after 72 h of incubation. TQ was more cytotoxic towards SiHa cells compared to cisplatin. Interestingly, TQ was less cytotoxic towards the normal cells (3T3-L1 and Vero). Cell cycle analysis performed by flowcytometer showed a significant increase in the accumulation of TQ-treated cells at sub-G1 phase, indicating induction of apoptosis by the compound. Apoptosis induction by TQ was further confirmed by Annexin V/PI and AO/PI staining. Significant elevation of p53 and down-regulation of the anti-apoptotic Bcl-2 protein was found in the treated cells, without any changes in the expression of the pro-apoptotic Bax protein. In conclusion, thymoquinone from N. sativa was more potent than cisplatin in elimination of SiHa cells via apoptosis with down-regulation of Bcl-2 protein. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24708/1/Thmoquinone%20from%20Nigella%20sativa%20was%20more%20potent%20than%20cisplatin%20in%20eliminating%20of%20SiHa%20cells%20via%20apoptosis%20with%20down.pdf Ng, Wei Keat and Saiful Yazan, Latifah and Ismail, Maznah (2011) Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein. Toxicology in Vitro, 25 (7). pp. 1392-1398. ISSN 0887-2333; ESSN: 1879-3177 10.1016/j.tiv.2011.04.030 |
| spellingShingle | Ng, Wei Keat Saiful Yazan, Latifah Ismail, Maznah Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title | Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title_full | Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title_fullStr | Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title_full_unstemmed | Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title_short | Thymoquinone from Nigella sativa was more potent than cisplatin in eliminating of SiHa cells via apoptosis with down-regulation of Bcl-2 protein |
| title_sort | thymoquinone from nigella sativa was more potent than cisplatin in eliminating of siha cells via apoptosis with down-regulation of bcl-2 protein |
| url | http://psasir.upm.edu.my/id/eprint/24708/ http://psasir.upm.edu.my/id/eprint/24708/ http://psasir.upm.edu.my/id/eprint/24708/1/Thmoquinone%20from%20Nigella%20sativa%20was%20more%20potent%20than%20cisplatin%20in%20eliminating%20of%20SiHa%20cells%20via%20apoptosis%20with%20down.pdf |