Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characteri...
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24703/ http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf |
| _version_ | 1848845108786495488 |
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| author | Al-Hetar, Khadega Yahya Abdullah Sekawi, Zamberi Shamsudin, Mariana Nor Neela, Vasantha Kumari |
| author_facet | Al-Hetar, Khadega Yahya Abdullah Sekawi, Zamberi Shamsudin, Mariana Nor Neela, Vasantha Kumari |
| author_sort | Al-Hetar, Khadega Yahya Abdullah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characterized 95 clinical isolates of ESBL-ExPEC collected over a one year period time from a tertiary hospital through Clermont dichotomous phylogenetic grouping, virulent gene profiling and ESBL production mechanisms. The highest frequency of E. coli isolation was observed among urine (40% / n=38), while the lowest was among tissue and peritoneal dialyses fluid (1.05% / n= 1). Phylogenetic grouping revealed that 42 (44.21%) isolates belonged to group D, while 26 (27.36%), 14(14.73%) and 13 (13.68) belonged to A, B2 and B1. All isolates were multi drug resistant, whereby 58.9% harboured CTX-M-1 and 22.10% CTX-M-9. None of the isolates carried CTX-M-2 or CTX-M-8 clusters. Eight (7.6%) isolates were positive for blaTEM-1 and two (1.9%) produced SHV-12 beta-lactamase. The CTX-M-9 cluster was found to be significantly (P=0.005) associated with pathogenic group. Majority of the isolates (48.42%) carried class 1 integrons, while 3 (3.15%) isolates showed positive for class 2-encodedintI2 integrase and no signal for class 3 integron. Virulence factors profiling identified iutA56 (58.94%) and kpsMII33 (34.73%) as frequent pathogenic determinants. Virulence studies on Caenorhabditis elegans showed that antibiotic susceptible E. coli strains have short life span than pathogenic or commensal ones. In conclusion, it is found that commensals are becoming multiple drug resistant and carries several virulent genes which warn the spread of drug resistance or virulent factors to the normal flora. |
| first_indexed | 2025-11-15T08:41:35Z |
| format | Article |
| id | upm-24703 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:41:35Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-247032017-11-17T02:05:02Z http://psasir.upm.edu.my/id/eprint/24703/ Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli Al-Hetar, Khadega Yahya Abdullah Sekawi, Zamberi Shamsudin, Mariana Nor Neela, Vasantha Kumari Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characterized 95 clinical isolates of ESBL-ExPEC collected over a one year period time from a tertiary hospital through Clermont dichotomous phylogenetic grouping, virulent gene profiling and ESBL production mechanisms. The highest frequency of E. coli isolation was observed among urine (40% / n=38), while the lowest was among tissue and peritoneal dialyses fluid (1.05% / n= 1). Phylogenetic grouping revealed that 42 (44.21%) isolates belonged to group D, while 26 (27.36%), 14(14.73%) and 13 (13.68) belonged to A, B2 and B1. All isolates were multi drug resistant, whereby 58.9% harboured CTX-M-1 and 22.10% CTX-M-9. None of the isolates carried CTX-M-2 or CTX-M-8 clusters. Eight (7.6%) isolates were positive for blaTEM-1 and two (1.9%) produced SHV-12 beta-lactamase. The CTX-M-9 cluster was found to be significantly (P=0.005) associated with pathogenic group. Majority of the isolates (48.42%) carried class 1 integrons, while 3 (3.15%) isolates showed positive for class 2-encodedintI2 integrase and no signal for class 3 integron. Virulence factors profiling identified iutA56 (58.94%) and kpsMII33 (34.73%) as frequent pathogenic determinants. Virulence studies on Caenorhabditis elegans showed that antibiotic susceptible E. coli strains have short life span than pathogenic or commensal ones. In conclusion, it is found that commensals are becoming multiple drug resistant and carries several virulent genes which warn the spread of drug resistance or virulent factors to the normal flora. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf Al-Hetar, Khadega Yahya Abdullah and Sekawi, Zamberi and Shamsudin, Mariana Nor and Neela, Vasantha Kumari (2011) Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli. African Journal of Microbiology Research, 5 (31). art. no. 5E0281D27330. pp. 5662-5668. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/5E0281D27330 |
| spellingShingle | Al-Hetar, Khadega Yahya Abdullah Sekawi, Zamberi Shamsudin, Mariana Nor Neela, Vasantha Kumari Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title | Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title_full | Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title_fullStr | Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title_full_unstemmed | Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title_short | Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli |
| title_sort | molecular characterization of extended-spectrum beta-lactimase (esbl) producing extra-intestinal pathogenic escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/24703/ http://psasir.upm.edu.my/id/eprint/24703/ http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf |